摘要
为研究高丛蓝莓对干旱胁迫的生理响应并筛选具有较强抗旱能力的品种,同时确定基于生理指标的隶属函数分析对于筛选蓝莓抗干旱品种的可行性,分别以北高丛蓝莓中的‘布里吉塔’、‘斯巴坦’以及南高丛蓝莓中的‘夏普蓝’、‘奥尼尔’4个品种为材料,在人工控水模拟水分胁迫条件下,测定叶片相对含水量、电导率、丙二醛含量、H2O2含量与O2-.产生速率、Fv/Fm以及叶片气孔特性等生理生化指标的变化,并采用隶属函数法进行综合评价。结果表明,随着干旱胁迫程度的加深,4个蓝莓品种叶片的相对含水量、Fv/Fm、气孔开放率和气孔大小均呈下降趋势,而相对电导率、丙二醛含量、H2O2含量与O2-.产生速率以及气孔密度则呈上升趋势。4个高丛蓝莓品种的抗旱性强弱顺序为奥尼尔>夏普蓝>布里吉塔>斯巴坦,大田干旱试验验证了基于生理指标的隶属函数分析对于蓝莓抗旱品种的筛选的准确性及可靠性。
Physiological characteristics of two northern（Brigitta,Spartan） and two southern highbush blueberry cultivars（Sharpblue,O’Neal）were investigated under water controlled conditions, as to unveil the possible mechanisms of highbush blueberry in response to drought stress and to screen the drought resistant cultivars. Meanwhile,the study also aimed to prove the possibility of using the Subordinate Function method based on physiological parameters to evaluate the drought-tolerance among the blueberry cultivars. The drought resistance capacity of all the four highbush blueberry cultivars were evaluated comprehensively by：The relative water content,content of the lipid peroxidation product （malondialdehyde,MDA）,membrane permeation,contents of hydrogen peroxide （H2O2） and superoxide radical（O2-.）,the maximum photochemical efficiency of PSⅡ（Fv/Fm）, stomatal characteristics of leaves. The results showed that drought stress decreased the relative water content,Fv/Fm,stoma open rate,stomatal length and stomatal width;Meanwhile,the relative conductivity,MDA content,H2O2 and O2-. contents and stomatal density were increased paralleled with the treatment intensity. The drought resistance abilities differed significantly among the four highbush blueberry cultivars,with the drought resistance capacity sequenced as following：O’Neal,Sharpblue, Brigitta , Spartan. The two-year field trial proved the Subordinate Function method based on physiological parameters could be used as a reliable method to screen the drought-tolerant blueberry cultivars.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第4期637-646,共10页
Acta Horticulturae Sinica
基金
浙江省重大科技专项(2008C12015-1)
浙江省科技创新团队项目(2011R09033-06)
关键词
高丛蓝莓
抗旱性
氧化胁迫
综合评价
隶属函数法
highbush blueberry
drought resistance
oxidative stress
comprehensive evaluation
subordinate function method
