摘要
采用SDS-PAGE法和近红外反射光谱法检测254份品种的HMW-GS和其中188份的沉淀值、蛋白质和湿面筋含量,研究甘肃春小麦育成品种和地方品种HMW-GS变异及品质效应。结果表明,共检测到21种亚基变异,46种亚基组成形式,育成品种亚基变异和组成形式不及地方品种丰富。Glu-1不同位点上,育成品种1、7+8和2+12分别为各自位点优势亚基(69.%、81.8%和86.0%),地方品种null、7+8和2+12为各自位点优势亚基(77.4%、88.7%和76.7%);育成品种1、14+15、5+10对蛋白质含量、沉淀值和湿面筋含量的效应值最高,地方品种2*、7+8和5+10对蛋白质含量的效应值最高,1、7+8和5+10亚基沉淀值和湿面筋含量的效应值最高。就优质亚基出现的频率而言,育成良品种Glu-A1位点品质改良效果较好。
Allelic variations of HMW-GS in 254 Gansu spring wheat landraces were studied by SDS-PAGE,and the quality including sedimentation,protein and wet gluten contents of 188 landraces were analyzed by near-infrared reflectance spectroscopy（NIRS） in this experiment.The results showed that 21 subunit types and 46 subunit compositions were detected in the Glu-1 loci,which implied that the landraces had much more diversities than that of the improved varieties.The subunits composition of 1（69.%）,7＋8（81.8%）,2＋12（86.0%） was the major type in the improved varieties,as well the subunits composition of null（77.4%）,7＋8（88.7%）,2＋12（76.7%） was the dominate combination in the landraces.The effect of 1,14＋15,5＋10 composition on quality of protein content,sedimentation and wet gluten content was the highest in the improved varieties,but the subunit combination 2＊,7＋8,5＋10 on quality of protein content was the highest in the landraces,and 1,7＋8,5＋10 on quality of sedimentation and wet gluten content was the highest.The frequency of fine subunit at Glu-A1 locus was higher than that at Glu-B1 and Glu-C1 loci in the improved varieties.
出处
《西北农业学报》
CAS
CSCD
北大核心
2012年第2期31-37,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
甘肃省科技厅科学事业费项目(QS031-C31-17)
甘肃省中青年科学基金项目(YS031-A21-009)
