摘要
【目的】通过研究转基因香石竹对土壤细菌群落的影响,为转基因香石竹的环境安全性评价提供依据。【方法】通过构建16S rDNA克隆文库,分析种植转基因和非转基因香石竹的土壤中细菌的群落结构组成。【结果】转基因和非转基因香石竹土壤中,共有的菌群有变形菌门(Proteobacteria)、浮霉菌门(Planctomycetes)、酸杆菌门(Acidobacteria),其中α-变形菌门、β-变形菌门、浮霉菌门为优势菌群;而在放线菌门(Actinobacteria)、疣微菌门(Verrucomicrobia)及未培养菌(Uncultured bacterium clone)等菌群存在部分差异。【结论】通过16S rDNA克隆文库方法揭示了转基因香石竹的土壤中细菌多样性十分丰富,其栽培对土壤细菌群落结构影响有限。
[Objective] We did this research to analyze the impact on the soil bacterial popula-tion of the transgenic carnation,which laid the foundation for the safety assessment of GM carnation.[Methods] Bacterial 16S rDNA gene clone libraries of GM and non-GM carnations were constructed,and the soil bacterial populations of these carnations were compared.[Results] The results showed Alphaproteobacteria,Betaproteobacteria,Planctomycetes and Acidobacteria were shared with GM and non-GM carnation.Some differences were found in Actinobacteria,Verrucomicrobia and uncultured bacterium clone.[Conclusion] The results indicated that high bacterial diversity was found in these GM carnation soil bacterial libraries,and the cultivation of genetically modified carnation did not have a significant impact on the soil bacterial community structure.
出处
《微生物学通报》
CAS
CSCD
北大核心
2012年第4期435-447,共13页
Microbiology China
基金
上海市科技兴农重点攻关项目(No.沪农科攻字2008第8-9号)
转基因生物新品种培育重大专项课题(No.2008ZX08012-001)
