摘要
目的探讨同种异基因大鼠骨髓间充质干细胞(MSCs)联合骨髓细胞(BMCs)输注对胰岛移植大鼠体内细胞因子水平的影响。方法制备SD大鼠DM模型,Wistar大鼠骨髓传代纯化获得MSCs。经静脉输注供体MSCs和/或BNCs后7d,糖尿病SD大鼠进行同种异体胰岛移植,观测胰岛移植物存活时间,检测白介素-2(IL-2)、干扰素-γ(IFN-γ)、白介素-4(IL-4)、白介素-10(IL-10)、白介素-6(IL-6)及转化生长因子-β1(TGF-β1)含量。结果预输注高浓度MCSs联合BMCs能显著延长移植胰岛的生存时间,平均为30d。该组大鼠体内IL-2、IFN-γ水平降低,IL-10和TGF-β1水平升高(P<0.05)。结论高浓度同种异基因大鼠MSCs联合BMCs输入体内后,使胰岛移植大鼠血清细胞因子发生显著变化,可能是诱导胰岛移植免疫耐受的原因之一。
Objective To investigate the influence of co-transfusion of mesenchymal stem cells and bone marrow cells before islet transplantation on cytokines in diabetic rat. lVlethofls Animal tndel of diabetes rnellitns was established. ~ from Wistar rats were purified through passage culture The donor rnesenchymal stem cells and/or bone marrow cells were injected into the recipient SD rats via veins, and diabetic SD rats received islet transplantation 7 days after the injectiorL Survival time of the islets grafts was observed, and the level of serum IL- 2,IFN-7, IL-4, IL-10 and TGF-/？I were detected. Results The pre-infnsion of ~ and ~ significantly prolonged allograft survival time（MST 30 d）. The contents of IL-2 and INF-y were reduced while the contents of IL-10 and TGF-131 were increased significantly after co-infnsion of high concentration of MSCs and BMCs. Omelusion The contents of cytokines can be chan_ged by co-transfusion of high-concentration MSCs and BMCs, which might be one of the reasons to induce pancreatic islets transplantation tolerance
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2012年第4期295-298,共4页
Chinese Journal of Diabetes
基金
国家自然科学基金资助项目(30570884)
广东省自然科学基金资助项目(05004760)
