摘要
目的:研究根皮苷对高脂导致氧化损伤的果蝇寿命及抗氧化能力的影响。方法:将2d龄果蝇随机分组,在高脂培养基下饲喂不同剂量(0、5、10mg/mL)的根皮苷,统计果蝇平均寿命,最高寿命及半数致死时间,分光光度计法测定25d果蝇超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量,并与模型组进行比较。结果:根皮苷组果蝇平均寿命、最高寿命及半数致死时间均显著高于高脂模型组,且不同剂量组之间呈现量效关系;SOD、CAT活性随根皮苷剂量升高而升高,MDA随根皮苷剂量升高而降低。结论:根皮苷对高脂膳食所导致的果蝇氧化损伤具有保护作用,能够明显延长果蝇寿命,增强SOD和CAT活性,降低MDA含量,具有抗氧化的作用。
Objective：The present study investigated the effect of phloridzin on the lifespan and antioxidant capacity of drosophila melanogaster upon oxidative stress induced by dietary fat.Methods：Phloridzin was administrated orraly to different groups of D.melanogaster fed with addition of 10% lard.The maximum life span,mean life span and 50% survival time were calculated.The activity of SOD and CAT and the content of MDA were analyzed.Results：The results showed that comparing with the model group,supplementation of phloridzin could significantly extend the lifespan of drosophila melanogaster.This was consistent with significant reduction in MDA content and increase in SOD and CAT activities in D.melanogaster.Conclusion：It was concluded that the antioxidant effect of phloridizn in D.melanogaster maintained on a high-fat diet was accompanied by up-regulation of CAT and SOD.
出处
《食品科技》
CAS
北大核心
2012年第4期195-198,共4页
Food Science and Technology
关键词
根皮苷
果蝇
抗氧化
高脂
phoridzin
drosophila melanogaster
antixoidant
fat
