摘要
目的采用乙酰丙酮分光光度法检测亚单位流感疫苗中的甲醛含量。方法采用乙酰丙酮分光光度法检测亚单位流感疫苗中的甲醛含量,对测定波长、乙酰丙酮、乙酸、乙酸铵浓度、保温时间、降温时间进行优化,并对方法进行验证。结果在500μl样品和4.5 ml乙酰丙酮溶液反应体系中,在0.125%乙酰丙酮、1.5%乙酸、25%乙酸铵条件下,40℃保温30 min,室温静置30 min后,在波长414 nm处测定甲醛含量,可获得最佳检测效果;在1～50μg/ml的浓度范围内标准曲线线性关系良好,回收率为101.9%,最低定量限为1μg/ml。结论应用乙酰丙酮分光光度法检测亚单位流感疫苗中的甲醛含量简便、快速、准确,灵敏度高,适用于实验室对甲醛含量的常规检测。
Objective To determine the formaldehyde content in influenza virus subunit vaccine by acetylacetone spectropho- tometry. Methods The formaldehyde content in influenza virus subunit vaccine was determined by acetylacetone spectrophotometry, based on which the detection wavelength, concentrations of acetylacetone, acetic acid and ammonium acetate as well as time for maintaining and lowering the temperature were optimized, and the method was verified. Results The optimal reaction system consisted of 500 μ1 of test sample and 4. 5 ml of acetylacetone. The optimal concentrations of acetylacetone, acetic acid and ammonium acetate were 0. 125%, 1. 5% and 25% respectively, while the optimal time for maintaining （at 40℃） and lowering the temperature were both 30 min, and the optimal detection wavelength was 414 nm. Under the optimized condition, the determination result was satisfactory. The standard curve showed good linearity within a concentration range of 1 - 50 p,g/ml, while the recovery rate and minimum detection limit were 101.9% and 1 μg/ml respectively. Conclusion Acetylacetone spectrophotometry was simple, rapid, accurate and sensitive for determination of formaldehyde content in influenza virus subunit vaccine, which was suitable for the routine determination of formaldehyde content in laboratory.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第4期510-512,共3页
Chinese Journal of Biologicals
关键词
甲醛
乙酰丙酮
流感疫苗
Formaldehyde
Acetylacetone
Influenza vaccine
