摘要
目的检测乳腺癌石蜡包埋组织中miR－195的表达，分析其与临床病理参数以及预后的关系。方法采用real-time PCR对88例乳腺癌患者原发肿瘤蜡块提取的RNA进行miR-195表达水平的测定，Mann Whitney-U非参数检验比较miR-195表达水平和乳腺癌临床病理参数的关系，采用Kaplan-Meier生存分析和Log-rank检验进行预后分析，Cox回归分析进行单因素、多因素分析。结果在高组织学分级、肿瘤大（134）、淋巴结转移及伴有脉管瘤栓的乳腺癌中miR-195表达水平相应地比低组织学分级（Z=-2．271，P=0．023）、肿瘤小（T1-2）（Z=-2．687，P=0．007）、无淋巴结转移（Z＝-1．967，P=0．049）及无脉管瘤栓（Z=-2．432，P=0．015）的乳腺癌下调。miR-195水平与雌激素受体／孕激素受体／HER-2表达、肿瘤TNM分期、是否复发、组织学类型及月经状态等未见明显关联（P〉0．05）。以中位数2^-ACt=0．270为界，Kaplan-Meier生存分析表明，miR-195表达水平与乳腺癌的无复发生存率（X^2=5．985，P=0．014）或总生存率（X^2=30．050，P=0．000）呈正相关。Cox回归多因素分析显示，miR-195（HR=0．040，95％CI：0．009-0．179，P=0．000）、组织学分级（HR=8．360，95％CI：2．25-31．00，P=0．001）、雌激素受体表达（HR=0．220，95％C1：0．06-0．80，P=0．022）及复发状态（HR=6．860，95％CI：l.70-27．60，P：0．007）是独立的预后因子。结论miR-195在乳腺癌组织的表达水平与组织学高级别、肿瘤大（13-4）、淋巴结转移、脉管瘤栓形成呈负相关。miR-195表达与乳腺癌预后相关。
Objective To explore the potential role of miR-195 on invasiveness and prognosis of breast cancer. Methods The RNA in formalin-fixed paraffin embedded （FFPE） of 88 breast cancer patients with primary tumors was extracted, and miR-195 levels were quantified by quantitative real-time polymerase chain reaction （real-time PCR）. The relationship of miR-195 levels and clinicopathologieal variables were assessed by Mann Whitney-U test. Recurrence-free survival and overall survival curves were derived from Kaplan-Meier estimates and the curves were compared by Log-rank tests. Cox regression analysis was used for multivariate analysis. All statistical tests were two-sided. Results The levels of miR- 195 in the breast cancer with histological high grade, tumor size of T3-4, lymph nodal involvement or vessel invasion were significantly down-regulated, compared with those of patients with histological low grade （ Z = - 2. 271, P = 0. 023 ）, tumor size of T1-2 （ Z = - 2. 687, P = 0. 007 ）, no lymph node metastasis （ Z = - 1. 967, P = 0. 049 ） and vessel invasion （ Z = - 2. 432, P = 0. 015 ）. In addition, no statistically significant difference （P 〉 0. 05 ） was identified between miR-195 levels and hormone receptors status, HER-2 expression, TNM stage, tumor types, recurrence and menstrual status. When considering 2^-ACt = 0. 270 （ median level） as cut-off value, Kaplan-Meier survival analysis indicated that patients with high miR- 195 level showed a positive association towards a longer survival, either recurrence-free survival （X^2 = 5.985, P=0. O14） or overall survival （X^2 = 30.05, P =0.000）. In a multivariate analysis, miR-195 expression on FFPE correlated significantly with outcomes of breast cancer （ HR = 0. 040, 95% CI：0. 009- 0. 179, P = 0. 000） and was independent of other prognostic factors. Conclusions It suggests that miR-195expression on FFPE is inversely correlated with histological high grade, bigger tumor size, lymph node involvement, vessel invasion. Furthermore
出处
《中华外科杂志》
CAS
CSCD
北大核心
2012年第4期353-356,共4页
Chinese Journal of Surgery
基金
福建省教育厅基金资助项目（JAlll20）
福建省自然科学基金资助项目（2006J0096）
关键词
乳腺肿瘤
肿瘤浸润
微RNAS
预后
Breast neoplasms
Neoplasm invasiveness
MicroRNAs
Prognosis
