摘要
为了建立分光光度法测定猪精子密度的标准方法,比较了不同波长(450、550、650 nm)下吸光度(A)、透射比(T)与精子密度(C)的关系。吸光度的检测上限随波长的增加而上升,分别为2.016亿/mL(450 nm)、2.24亿/mL(550 nm)和2.35亿/mL(650 nm),但重复测定的稳定性下降。透射比的观测值随稀释倍数增加,重复测定的稳定性降低,当稀释倍数大于10倍(450 nm)、6倍(550 nm)、4倍(650 nm)时重复测定的观察值之间开始出现差异。试验结果表明,450 nm为最适检测波长,猪精子密度与吸光度、透射比分别呈三次函数和幂函数回归关系,回归方程分别为:C450=0.48A3-0.76A2+0.67A-0.066(R=0.951)和C450=1.657T-0.1068(R=0.940)。
To provide the necessary standardization for procedures of sperm concentration estimation, a spectrophotomeric method has been established to determine the sperm concentration of boar seme. Wavelengths of 450 nm,550 nm,and 650 nm have been compared,and 450 nm was found to be the most sensitive and reliable. A logarithmic relationship between sperm concentration（C） and optical absorbance （A） or spectrophotomeric transmittance （T）was observed for sperm concentration. The regression equation for the standard curve at 450 nm was C~,,,=0.48A3-O.76A2＋O.67A-O.O66CR=O.951）and C~,=l.657-a1068=0.940）.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第5期103-105,108,共4页
Guangdong Agricultural Sciences
基金
重庆市畜牧科学院基本科研业务费专项
重庆市科委专项资金(10608)
关键词
猪
精子密度
分光光度法
swine
sperm concentration
spectrophotomeric method
