摘要
目的研究单发胃癌和食管/胃双原发癌中胃癌组织CDH1基因启动子区CpG岛甲基化变化及其临床意义。方法应用甲基化特异性PCR法,检测62例单发胃癌、30例食管/胃双原发癌中胃癌组织、癌旁组织及18例正常胃组织CDH1基因甲基化情况。结果单发胃癌和食管/胃双原发癌中胃癌组织CDH1基因甲基化率分别为61.3%和76.7%,癌旁组织分别为22.6%和23.3%,正常胃组织为0。单发胃癌和食管/胃双原发癌患者癌组织CDH1基因甲基化率与癌旁组织、正常组织比较,差异均有统计学意义(P<0.05),癌旁组织CDH1基因甲基化率与正常组织比较,差异均无统计学意义(P>0.05)。单发胃癌与食管/胃双原发癌中胃癌组织CDH1基因甲基化阳性率比较,差异无统计学意义(P>0.05)。单发胃癌和食管/胃双原发癌患者CDH1基因甲基化与性别、年龄、烟酒史和上消化道肿瘤家族史均无关(P>0.05)。结论 CDH1基因甲基化在单发胃癌和食管/胃双原发癌胃癌中是一常见的表观遗传学事件,可能是一个独立的危险因素。
Objective To study the changes of CDH1 gene promoter CpG island methylation and its clinical signifi- cance in patients with single gastric cancer （SGC） and gastric cancer （GC） in esophagus/stomach double primary cancers （ ES- DC）. Methods 62 patients with SGC, 30 patients with GC of ESDC and 18 healthy persons were assigned to be checked CDH1 gene Methylation of cancerous tissues, adjacent non - cancerous tissues and normal health gastric tissues by methylation specific polymerase chain reaction （MSP）. Results The rates of CDH1 gene Methylation in the cancerous tissues were 61.3% and 76. 7% respectively, and 22. 6% and 23. 3% respectively in adjacent non -cancerous tissues in patients with SGC and GC of ESDC, and 0 in normal gastric tissues. There were significant differences between the cancerous tissues and adjacent non - can- cerous tissues, normal gastric tissues （P all 〈 0.05）. And the differences between adjacent non - cancerous tissues and normal tissues was not significant （ P all 〉 0.05 ）. The difference of positive rate of CDH1 gene Methylation between cancerous tissues in SGC and those in GC of ESDC was not statistically significant （ P 〉 0.05 ）. There were no relationship between CDH1 gene methylation and sex, age, tobacco - alcohol history and family history of upper gastrointestinal cancer （MGIC） （Pall 〉 0. 05 ）. Conclusion CDH1 gene methylation in SGC and GC in ESDC is a common epigenetic genetics event. It may be an in- dependent risk factor.
出处
《中国全科医学》
CAS
CSCD
北大核心
2012年第9期1006-1009,共4页
Chinese General Practice
基金
河北省2010年医学科学研究重点课题计划(20100361)
关键词
胃肿瘤
双原发癌
CDH1
甲基化
Gastric neoplasms
Double primary cancer
CDH1
Methylation
