摘要
目的检测MYH14基因在先天性小耳畸形中的表达情况,进而探讨基因表达的异常与先天性小耳畸形发病之间的关系。方法收集先天性小耳畸形患者的残耳软骨组织及细胞为研究对象,非耳畸形患者的正常耳软骨组织及细胞作为对照。分别提取RNA,利用实时荧光定量PCR技术对各组的基因表达情况进行检测。结果软骨组织实验组与对照组在MYH14基因的表达存在差异,且具有统计学意义。结论 MYH14基因的表达异常可能与小耳畸形的发病相关。
Objective： To study the expression of MYH14 gene in congenital microtia and to discuss the relationship between the expression of MYH14 and the etiology of the disease.Methods： To collect the residual ear cartilage and chondrocyte of microtia as the research object;normal ear cartilage and chondrocyte of patients without ear malformation as control.Using the real-time quantitative PCR to test the RNA expression level of MYH14 gene between each group in ear cartilage and chondrocyte.Results：In the cartilage,RNA expression in MYH14 of experimental group was higher than control group with significant difference.Conclusions： It suggested that the abnormal expression of MYH14 might relate to the pathogenesis of micrtia.
出处
《中国优生与遗传杂志》
2012年第4期13-16,共4页
Chinese Journal of Birth Health & Heredity
基金
国家自然科学基金资助项目(30901568)
