摘要
松材线虫病又称松树枯萎病,是由病原松材线虫（Bursaphelenchus xylophilus）引起的一种毁灭性森林病害。随着国际贸易日益频繁,松材线虫随木包装以及木材的进出口,反复传播的可能性越来越大（张星耀等,2003）,而传统的熏蒸、热处理和杀线虫剂处理效率低、易造成环境污染（陈劲松等,
In this paper, we employed the pulsed magnetic field （PMF） processing to investigate the effect of different pulse rise time and treatment time on the survival, reproduction, ceUulase activity and surface characteristics of the pine wood nematode （PWN）. The results showed that the wider pulse and the longer treatment time were able to reduce the survival, and inhibit the cellulase activity and reproduction of PWN. The 4th instar and females had a strong resistance to the pulsed magnetic field. With pulse rise time of 10 ms and the treatment of 60 rain, the adjusted mortality of PWN was 100%. After the PMF treatment, the cellulase activity of PWN increased within a short time, and then declined with the treatment time. The observation with a scanning electron micrograph （SEM） revealed that there was no significant change in morphology of PWN, but the quantity of rod shaped bacteria carried by PWN was reduced.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2012年第3期136-142,共7页
Scientia Silvae Sinicae
基金
国家自然科学基金项目(30671687)
关键词
脉冲磁场
脉冲前沿宽度
纤维素酶活性
死亡率
松材线虫
pulsed magnetic field
pulse rise time
cellulase activity
mortality rate
pine wood nematode
