摘要
目的:观察蝎毒多肽(PESV)对人白血病NOD/SCID小鼠髓外浸润模型体内uPA、uPAR、MMP2、MMP9表达的影响,探讨PESV干预急性白血病髓外浸润传变的机制。方法:首先选取急性白血病患者骨髓单个核细胞注入经过铯-137源照射的NOD/SCID小鼠体内,建立人白血病NOD/SCID小鼠髓外浸润模型;对实验小鼠随机分组,用Real-time PCR及Western Blot检测PESV治疗后小鼠体内MMP2、MMP9 mRNA和蛋白表达水平,用ELISA法检测小鼠血清uPA及uPAR表达水平。结果:PESV能够抑制模型小鼠体内MMP2、MMP9 mRNA及蛋白水平表达,能够抑制uPA及uPAR过度表达,其抑制效果与PESV浓度具有相关性。结论:PESV通过干预细胞外基质降解机制,阻抑急性白血病髓外浸润传变进展。
Objective： To investigate the peptide extracts from scorpion venom（PESVs） interfering in urokinase-type PA（uPA） and its receptor,the matrix metalloproteinase2（MMP2）,matrix metalloproteinase9（MMP9） expression in leukemia-NOD/SCID Mice,and to research the mechanism of PESV inhibiting the multiplication and infiltration of leukemic cells.Methods： To begin with,bone marrow mononuclear cells of leukemia patients were injected into NOD/SCID mice,irradiated 270 cGy on body by137Cs beforehand,in order to establish the animal model of outer marrow infiltration of human leukemia in NOD/SCID mice.What＇s more,randomly to divide the mice of model establishment into four groups.Group I-III were respectively cured by the peptide extract from scorpion venom（PESV） with different concentrations and Group IV was the model group injected by the physiological saline water.Group V was taken as control.Finally,To kill these mice after four weeks and to use Real-time PCR method to examine MMP2,MMP9 mRNA expression in mice,simultaneously to use western blot method to examine MMP2,MMP9 protein expression.Furthermore,to use ELISA method to examine uPA and uPAR expression in mice＇s blood serum.Results： The PESV,anticancer peptide in east asian pliers scorpion poisonous,has good inhibitory role in leukemic cell multiplication and infiltration,which effectively suppress the MMP2,MMP9 mRNA and protein expression and inhibit the uPA and its receptor in leukemia-NOD/SCID mice.The effect of inhibiting them was related to PESV concentration.Conclusion： The PESV has good role in inhibiting acute leukemic cell extramedullary infiltration and transmutation by influencing ECM degradation system.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第4期1106-1110,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.30672743)
天津市应用基础研究项目(No.07JCYBJC11500)~~
关键词
蝎毒多肽
急性白血病髓外浸润
传变机制
分子机制研究
Peptide extract from scorpion venom
Acute leukemic cell extramedullary infiltration
Mechanism for transmutation
Research of molecular mechanism
