摘要
目的：观察薯蓣皂苷对离体大鼠主动脉环的舒张作用。方法：采用离体器官水浴法,分为3组,分别为对照组、完整内皮组和去内皮组,观察薯蓣皂苷（0.1~3.2 mmol/L）对苯肾上腺素（PE）、氯化钾（KCl）预收缩的大鼠主动脉环的作用,进行组间比较,并观察薯蓣皂苷对离体血管作用的内皮依赖性。结果：（1）对PE预收缩内皮完整血管环,薯蓣皂苷在0.1 mmol/L即产生显著舒张作用（vs对照组P〈0.01）且有剂量依赖性;（2）对PE预收缩去内皮血管环,薯蓣皂苷在1.6 mmol/L产生舒张作用但效果不显著（P〉0.05）,在3.2 mmol/L产生舒张作用（P〈0.05）;（3）在相同浓度薯蓣皂苷（0.1~0.8 mmol/L）条件下,PE对内皮完整组舒张程度显著高于去内皮组（P〈0.05）,表明薯蓣皂苷对PE预收缩的血管环舒张作用有一定的内皮依赖性;（4）对KC1预收缩内皮完整血管环,薯蓣皂苷在0.8 mmol/L产生显著舒张作用（vs对照组P〈0.01）且有剂量依赖性;（5）在KC1预收缩的去内皮血管环,薯蓣皂苷1.6 mmol/L产生舒张作用但效果不显著（P〉0.05）,薯蓣皂苷3.2 mmol/L产生舒张作用（P〈0.05）;（6）在相同浓度薯蓣皂苷（0.8~1.6 mmol/L）条件下,薯蓣皂苷对内皮完整主动脉环的舒张作用显著高于去内皮组（P〈0.05）,表明薯蓣皂苷对KC1预收缩的血管环舒张作用有一定的内皮依赖性。结论：薯蓣皂苷通过内皮和非内皮作用机制舒张血管,并具有剂量依赖性。
Objective： To observe the effects of dioscin on aortic vascular rings isolated from rat.Methods： The aortic vascular rings were perfused with water bath method and divided into 3 groups,namely as control group,complete endothelial group and none endothelial group.The effects of dioscin（0.1~3.2 mmol/L） on the vascular ring pre-contracted with phenylephrine（PE）,or potassium chloride（KCl） were observed,under the condition of endothelium intact and without endothelium.Results：（1）Under the conditions of vascular endothelial integrity,dioscin produced a significant vasodilation from 0.1 mmol/L（vs control group P0.01） on the vascular rings pre-contraction with PE,and possessed a dose-dependent vasodilation function;（2） Under the conditions of none vascular endothelial,dioscin produced diastolic function but not significant in 1.6 mmol/L（P0.05） on the vascular rings pre-contraction with PE,and produced vasodilation function in 3.2 mmol/L（P0.05）;（3） Under the conditions of the same concentration of dioscin（0.1~0.8 mmol/L）,the degree of vasodilation was significantly higher in the complete vascular endothelial group than that in the none vascular endothelial one（P0.05） on the vascular rings pre-contraction with PE,which shown that the dioscin possessed a endothelial – dependent vasodilation function on PE pre-contraction of vascular rings;（4） Under the conditions of vascular endothelial integrity,dioscin produced a significant vasodilation from 0.8 mmol/L（vs control group P0.01） on the vascular rings pre-contraction with KCl,and posessed a dose-dependent diastolic function;（5） Under the conditions of none vascular endothelial,dioscin produced diastolic function but not significant in 1.6 mmol/L（P0.05） on the vascular rings pre-contraction with KCl,and produced diastolic function in 3.2 mmol/L（P0.05）;（6） Under the conditions of the same concentration of dioscin（0.8-1.6 mmol/L）,the degree of vasodilation was significantly higher in the complete v
出处
《天津医科大学学报》
2012年第1期24-26,共3页
Journal of Tianjin Medical University
