摘要
目的探讨胆管细胞癌中潜在的肿瘤干样细胞标记CD133的表达状况及其与临床病理的关系。方法采用兔抗人CD133抗体和非生物素二步法免疫组织化学技术检测53例胆管细胞癌、30例癌旁组织和20例正常胆管组织标本中CD133的表达状况并分析其与患者年龄、性别、肿瘤部位、组织学分级以及临床分期的关系。结果胆管细胞癌的CD133表达较癌旁组织和正常胆管组织明显升高(P<0.001)。CD133表达与年龄、性别及肿瘤部位无关(P值分别为0.779、0.256和0.409),但与癌组织学分级(P=0.014)及临床分期(P=0.028)呈正相关。Kaplan-Meier生存分析提示CD133表达与胆管细胞癌的预后相关(P=0.004)。结论 CD133表达可能对胆管细胞癌的发生、演化和进展具有一定的作用,并可能成为辅助胆管细胞癌诊断及预后评价的一个新标记物。
Objective To investigate the expression of CD133 protein,and the possible relationship between CD133 expression and clinicopathological indices in cholangiocellular carcinoma.Methods Detecting CD133 protein expression in formalin-fixed,paraffin-embedded 53 cholangiocellular carcinomas,30 adjacent non-neoplastic tissues and 20 normal bile ducts and analysing their relationships to patient＇s age,sex,site,histological grade,and clinical stage were carried out by two-step non-biotin immunohistochemical method.Results CD133 expression in cholangiocellular carcinoma was significantly higher than that in adjacent non-neoplastic or normal bile duct tissues（P0.001）.Carcinomas with positive expression of CD133 were significantly corresponding to the histological grade（P=0.014）and clinical stage（P=0.028）,but not to the age（P=0.779）,sex（P=0.256）and site（P=0.409）,respectively.By Kaplan-Meier survival analysis,the expression of CD133 was significantly correlative with the median survival time（P=0.004）.Conclusions The expression of CD133 may be associated with the carcinogenesis of cholangial epithelia,and the differentiation,development and the prognosis of the cholangiocarcinoma,thus may become a new adjunct diagnostic and prognostic markers in cholangiocelluar carcinoma.
出处
《中华临床医师杂志（电子版）》
CAS
2012年第1期I0001-I0004,共4页
Chinese Journal of Clinicians(Electronic Edition)
关键词
胆管肿瘤
病理学
免疫组织化学
Bile duct neoplasms Pathology Immunohistochemistry
