摘要
目的分析解脲脲原体(Uu)和血清抗精子抗体(AsAb)对精液质量的影响。方法选取62例男性不育患者,以Uu选择培养基对其分泌物培养鉴定,以酶联免疫吸附试验(ELISA)检测其精浆和AsAb、IgM、IgG和IgA,以精液分析仪进行精子密度、存活率、a+b级活力和畸形率分析;然后根据结果不同,分为Uu阴性和Uu阳性不育组、AsAb阴性和AsAb阳性不育组,分别加以比较。结果 Uu阳性不育组精子畸形率为(25.62±17.90)%,较Uu阴性不育组的(12.81±8.46)%明显增高(P<0.05),而两组间精子密度、精子存活率和精子活力差异无统计学意义(P>0.05);AsAb阳性不育组精子密度、精子存活率和精子活力分别为(37.21±42.95)×106/mL、(23.82±20.30)%、(11.79±12.62)%;AsAb阴性不育组分别为(90.01±83.52)×106/mL、(52.25±30.10)%、(35.28±23.46)%,两组比较差异有统计学意义(P<0.05),而两组间精子畸形率差异无统计学意义(P>0.05);AsAb各亚型间精子存活率和精子活力差异有统计学意义(P<0.05),而各亚型间精子畸形率和精子密度差异无统计学意义(P>0.05)。结论 Uu和AsAb严重影响精液质量,这对男性不育类型的鉴别诊断和选择治疗方案具有重要意义。
Objective To study the influence of Uu and serum antisperm antibodies （AsAb） on the main pa rameters of semen. Methods 62 cases of male infertility were selected and their secretions were cultured for identifi- cation by selective culture medium with Uu. The seminal plasma and serum AsAb, IgM, IgG and IgA were detected by ELISA. The sperm density, sperm survival rate, sperm a＋ b activity and sperm deformity rate were detected by the semen analyzer. According to the detected results, the patients were divided into the Uu positive infertile group and Uu negative infertile group and the comparison among the semen items in the two groups were made. Results The sperm deformity rate in the Uu positive infertile group was 25.62% ± 17. 90% and significantly higher than （12.81± 8.46）% in the Un negative infertile group （P〈0.05）, and the sperm density, survival ratio and motility in the two groups had no statistical difference （P〉0.05）. The sperm density,survival ratio and motility in the AsAb positive infertile group were （ 37.21± 42.95） × 106/mL, （ 23.82 ± 20. 30 ） % and （ 11.79 ± 12.62 ） %, which in the AsAb negative were （ 90.01 ± 83. 52） × 106/mL, （52. 25 ± 30. 10） % and （35.28 ± 23.46）%, showing statistical difference between the two groups（P〈0.05）. But the sperm malformation rate between the two groups had no sig- nificant difference （P〉0.05）. The sperm survival ratio and the motility among various AsAb subtypes had statistic difference （P〈0.05） ,and the sperm malformation rate and sperm density among various AsAb subtypes showed no statistical difference （P〉0.05）. Conclusion Uu and AsAb seriously affect the semen quality,which have important significance on differential diagnosis and treatment options in male sterile types.
出处
《检验医学与临床》
CAS
2012年第7期813-815,共3页
Laboratory Medicine and Clinic
