摘要
目的观察自体骨髓单核细胞移植对大脑中动脉栓塞（MCAO）大鼠脑梗死体积、血脑屏障破坏程度及行为学评分等的影响，评价骨髓单核细胞移植对脑梗死的治疗价值。方法将72只成年雄性SD大鼠按完全随机数字表法分为假手术组、模型组、生理盐水组及骨髓单核细胞移植组，后三组采用线栓法制作MCAO大鼠模型。采用梯度离心法分离大鼠股骨骨髓腔中的单核细胞，并通过流式细胞术检测CD45＋细胞比例。将分离得到的自体骨髓单核细胞经颈静脉途径移植到骨髓单核细胞移植组大鼠体内，生理盐水组大鼠给予等体积的生理盐水注射。造模后72h时伊文氏蓝染色及脑组织含水量检测显示血脑屏障的破坏情况，造模后1d、3d、7d时通过Zea—Longer评分评价大鼠神经功能变化，最后一次Zea—Longer评分结束后TTC染色检测大鼠脑梗死体积。结果流式细胞术检测发现骨髓单核细胞中CD45＋细胞的比例为91．2％。脑梗死体积、伊文氏蓝含量及脑组织含水量在模型组与生理盐水组中均较高，且相互比较差异无统计学意义（P〉0．051；骨髓单核细胞移植组脑梗死体积、伊文氏蓝含量及脑组织含水量均明显下降．较模型组与生理盐水组差异均有统计学意义（P〈0．05）。Zea．Longer评分显示，除假手术组外，其余大鼠均有不同程度的神经功能缺损：造模后7d时骨髓单核细胞移植组神经功能评分明显改善，与模型组及生理盐水组比较差异均有统计学意义（P〈0．05）。结论骨髓单核细胞分离过程简单，利用其移植治疗脑梗死可以显著减轻脑损伤程度并促进神经功能恢复，对脑梗死具有很高的治疗价值。
Objective To investigate the therapeutic role of autologous bone marrow mononuclear cells （BM-MNCs） in cerebral infarction by detecting the infarct volume, integrity of blood-brain barrier （BBB） and functional recovery in rats with middle cerebral artery occlusion （MCAO）. Methods Seventy-two adult male SD rats were randomly divided into sham-operated group, vehicle group, normal saline treatment group and BM-MNC treatment group. MCAO in rats of the later 3 groups was induced by occlusion of the right middle cerebral artery using an intra luminal filament technique. BM-MNCs, selected from their femur, were isolated with gradient centrifugation, and the proportion of CD45 ＋ cells was detected by flow cytometry before infusion. BM-MNCs were re-injected by jugular vein infusion. Infarct volumes were determined with 2,3,5-triphenyltetrazolium chloride （TTC） staining; the content of Evans blue and brain water content were detected to evaluate the damage of BBB; in addition, neurological deficits were also assessed by Zea-Longer tests. Results Flow cytometry revealed that the proportion of CD45 ＋cells in BM-MNCs was 91.2%. The infarct volumes, contents of Evans blue and brain water in rats of BM-MNC treatment group were significantly decreased as compared with those in rats of the normal saline treatment group and vehicle group （P〈0.05）; however, no significant difference of those was noted between rats of the normal saline treatment group and vehicle group （P〉0.05）. Except for the sham-operated group, all the other rats had neurological deficits of varied degrees; the neurological deficits in rats of the BM-MNC treatment group were obviously improved as compared with those in rats of the vehicle group and normal saline treatment group （P〈0.05）. Conclusion The separation of BM-MNCs is simple; BM-MNCs can significantly reduce the extent of brain injury and promote recovery of neurological function in rats with cerebral infarction, which indicate that BM-MNCs may have high therapeutic
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2012年第4期353-357,共5页
Chinese Journal of Neuromedicine
关键词
脑梗死
骨髓单核细胞
细胞移植
Cerebral infarction
Bone marrow mononuclear cell
Cell transplantation
