摘要
目的建立生物样品中甲醇的气相色谱测定方法。方法取生物样品于顶空瓶中,加入氯化钠、水,在60℃水浴气-液平衡20 min后,气相色谱法测定,外标法定量。结果在0.10 mg~1.00 mg样品添加水平范围内,方法的回收率在94.7%~102%,在不同样品测定中,方法的相对标准偏差为3.5%~6.3%。结论该方法操作简便,稳定可靠,定量准确,适用于生物样品中甲醇的测定。
Objective To establish gas chromatographic method for determination of methanol in biological samples.Methods The biological samples including blood,urine and content of stomach were placed in head-space vial,and added sodium chloride and water for gas-liquid equilibrium under 60℃ water-bath for 20 minutes,external standard quantification was used for definite quantitative determination.Results For spiked level of 0.10-1.00mg,the recovery rate of the method was in the range of 94.7%-102%,with the relative standard deviations of 3.5%-6.3%.Conclusion This method is simple,reliable,accurate and suitable for the determination of methanol in biological samples.
出处
《预防医学情报杂志》
CAS
2012年第3期234-235,共2页
Journal of Preventive Medicine Information
关键词
气相色谱
生物样品
甲醇
gas chromatographic
biological samples
methanol