摘要
目的研究二苯乙烯苷(TSG)对过氧化氢(H2O2)诱导损伤的人脐静脉内皮细胞单核细胞趋化蛋白1(MCP-1)表达的影响。方法体外培养人脐静脉内皮细胞,分为空白对照组、H2O2组(200μmol/L H2O2)、辛伐他汀组(5μmol/L辛伐他汀+200μmol/L H2O2)、TSG组(1μmol/L TSG+200μmol/L H2O2),按分组处理24 h细胞后,运用逆转录聚合酶链式反应和酶联免疫吸附试验分别检测MCP-1 mRNA与其蛋白的表达。结果 200μmol/L的H2O2作用内皮细胞24 h后,MCP-1的mRNA和蛋白水平均较空白对照组显著升高,P<0.01。TSG预处理细胞后,MCP-1的mRNA和蛋白水平较H2O2组降低,差异有显著性(P<0.05)。结论 TSG能抑制H2O2诱导损伤的人脐静脉内皮细胞MCP-1的表达。
Objective To study the effect of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside（TSG） on expression of monocyte chemoattractant protein-1（MCP-1） induced by H2O2 on human umbilical vein endothelial cells（HUVEC）.Methods Endothelial cells were cultured in vitro and divided into group of control,H2O2（200 μmol/L）,simvastatin（5 μmol/L simvastatin plus 200 μmol/L H2O2） and TSG（1 μmol/L TSG plus 200 μmol/L H2O2）,after treated for 24 h,the expression of MCP-1 mRNA and protein were detected by reverse transcriptase polymerase chain reaction（RT-PCR） and enzyme-linked immunosorbent assay（ELISA） respectively.Results Treating endothelial cells with 200 μmol/L H2O2,the expression of MCP-1 mRNA and protein level was significantly higher（P0.01）.TSG can inhibit the expression of MCP-1 mRNA and protein level induced by H2O2 on endothelial cells（P0.05）.Conclusions TSG can inhibit the expression of MCP-1 induced by H2O2 in human umbilical vein endothelial cells.
出处
《中南医学科学杂志》
CAS
2012年第1期14-16,54,共4页
Medical Science Journal of Central South China
基金
国家自然科学基金(30800474)资助
湖南省科技厅项目(2010SK3037)资助
湖南省教育厅重点项目(11A104)资助
湖南省研究生创新项目(CX2010B385
CX2011B378)资助
