摘要
试验旨在研究TLR2、4/MyD88通路相关分子mRNA在炎症过程中的作用机制及相互关系。诱导急性结肠炎小鼠模型,分别于造模前、造模后1、3、5、7 d各处死6只小鼠,并采用实时荧光定量RT-PCR检测结肠组织中TLR2、TLR4、MyD88、NF-κB、TNF-α和IL-10 mRNA的表达量变化。结果表明,与造模前比较,TLR2mRNA在造模后3、5 d的表达量显著增加(P<0.05),造模后7 d时的表达具有极显著差异(P<0.001);TLR4在造模后1 d就显著上调(P<0.05),在造模后3、5、7 d更是异常高表达(P<0.001);MyD88和NF-κB mRNA的表达量都在造模后5、7 d迅速增加(P<0.01);TNF-α在造模后各阶段(除造模后1 d外)均出现高表达(P<0.01或P<0.001);IL-10在造模后1 d的转录水平急剧上升(P<0.001),此后则逐渐回落,但其表达水平高于造模前。TLR2/4可能通过正调节MyD88、NF-κB和TNF-α,负调节IL-10等关键节点分子的mRNA表达水平来参与炎症反应。
To study the association of TLR2,4/MyD88 pathway molecules mRNA in inflammation,a mouse model with acute colitis was induced.Six mice were killed respectively for 0,1,3,5 and 7 day old.The mRNA expression levels of TLR2,TLR4,MyD88,NF-κB,TNF-α and IL-10 were detected by quantitative real-time reverse-transcriptase polymerase chain reaction.The results showed that,compared with the 0 day group,the transcription level of TLR2 mRNA was significantly high on 3,5 days(P〈0.05),and its expression was a significantly difference on 3,5 day old(P〈0.001);1 day old after were induced,the expression of TLR4 was up-regulated significantly(P〈0.05),and it was more abnormal expression on 3,5 and 7 day old(P〈0.001);MyD88 and NF-κB mRNA were increased rapidly on 5,7 day old(P0.01);it showned that TNF-α was high expression at all stages(excepting one day old)(P0.01 or P〈0.001);the transcription level of IL-10 rised sharply in the first day(P〈0.001),since then it reduced gradually but its expression level were still higher than the previous of induction.TLR2/4 possibly through up-regulate mRNA expressions of MyD88,NF-κB and TNF-α,but down-regulate the transcription level of IL-10 mRNA to contribute to the inflammatory response.
出处
《饲料博览》
2012年第3期5-9,共5页
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