摘要
目的 探讨子宫内膜癌微卫星不稳定性 (microsatelliteinstability ,MI)的发生率及其与各临床病理参数和雌激素受体 (ER)、孕激素受体 (PR)水平的关系。方法 采用聚合酶链反应 (PCR)、变性聚丙烯酰胺凝胶电泳等技术 ,在 8个位点上对 40例散发性子宫内膜癌病人进行MI测定。以免疫组织化学法检测了 2 7例病人的ER、PR水平 ,并比较MI与临床病理参数和ER、PR水平的关系。结果9例 (2 3% )在两个或两个以上的位点显示为MI阳性 ,称为复制错误 (replicationerror,RER)阳性 ,余 31例 (77% )为RER阴性 ,即表现为仅 1个位点有MI或 8个位点均无MI。 9例RER阳性者均为子宫内膜样癌 ,非子宫内膜样癌中无MI阳性者 ;36例子宫内膜样癌中 ,中、低分化者RER阳性率为 5 0 % (5 /10 ) ,明显高于高分化者的 15 % (4/ 2 6 ) ,差异有显著性 (P <0 .0 5 ) ;MI与临床分期和子宫肌层浸润深度无关 (P >0 .0 5 )。 2 7例检测ER、PR水平的病人中 ,19例 (70 % )表现为ER、PR均阳性 ;子宫内膜样癌中RER阳性者ER、PR均阳性率为 6例中 3例 (3/ 6 ) ,明显低于RER阴性者的 84% (16 / 19) ,差异有显著性 (P <0 .0 5 )。结论 MI是子宫内膜癌发生、发展的机理之一 ,与病理分化程度、组织学类型及ER、PR水平有关。
Objective To assess the prevalence of microsatellite instability in a series of endometrial carcinomas as well as to define the clinicopathologic features and estrogen receptor (ER) and progesterone receptor (PR) status associated with microsatellite instability (MI). Methods We examined 40 cases for replication error (RER) using polymerase chain reaction, polyacrylamide gel electrophoresis and silver stain at 8 microsatellite loci. Immunohistochemical staining of 27 paraffin sections was performed using antibodies to ER and PR. Finally, MI was compared with the clinicopathologic characteristics as well as ER and PR status. Results MI was observed in 9 (23%) at two or more loci, which is defined as RER positive phenotype. The 9 RER positive cases were all endometrioid type. Four non endometrioid tumors failed to show MI in any locus. RER positive phenotype was more frequent in poorly differentiated (50%) than in well differentiated tumors (15%). We found no significant correlation of RER with stage and depth of invasion. Of 27 carcinomas, 19 (70%) showed homogeneous positive staining for ER and PR. ER and PR positive staining occured more frequently in RER negtive endometrioid cancers than in RER positive cases. Conclusions MI is one of the molecular mechanisms of a subset of endometrial carcinomas. Its frequency is associated with tumor grade, histological type and ER or PR status.
出处
《中华妇产科杂志》
CSCD
北大核心
2000年第5期273-276,共4页
Chinese Journal of Obstetrics and Gynecology
关键词
子宫内膜肿瘤
DNA卫星
受体
雌激素
受体
孕酮
Endometrial neoplasms
DNA,satellite
Receptors,estrogen
Receptors,progesterone
