摘要
目的评价12种肿瘤相关抗原(TAA)微阵列在早期肺癌中的诊断价值。方法采用酶联免疫吸附实验(ELISA)检测174例肺癌患者和92名正常人群血清中的12种TAAs的抗体水平,应用流行病学筛检试验的指标对TAAs微阵列的结果进行评价;用ROC曲线来确定最佳的微阵列组合,并用受试者工作特征曲线(ROC)下面积来判断其诊断价值。结果每种TAA的抗体水平在肺癌患者血清中的阳性率均高于正常对照组(P<0.05);单个TAA检测的灵敏度在12.6%～27.0%;12种TAAs微阵列检测的灵敏度增加到72.4%,特异度达到91.3%,正确指数为0.637,阳性预测值为94.0%,阴性预测值为63.6%,ROC曲线下面积为0.819。结论联合12种TAAs微阵列对肺癌具有较高的诊断价值,可提高肺癌的检出率,有助于肺癌早期诊断。
Objective To evaluate the value of a panel of twelve tumor-associated antigens（TAAs） mini-array in the early diagnosis of lung cancer.Methods Totally 174 lung cancer patients and 92 healthy individuals were selected.The enzyme-linked immunosorbent assay（ELISA） was performed to detect all TAA antibodies in the serum.The diagnostic value was evaluated by epidemiological methods in screening.Then receiver operating characteristic curve（ROC curve） was used to determine the best combination of TAAs mini-array and judge its diagnostic value.Results In serum samples from lung cancer patients,the positive rates of TAAs were significantly higher than those in the healthy controls（P〈0.05）.The sensitivity in lung cancer diagnosis using single antigen was low.The sensitivity of Calnuc was the highest（27.0%） and that of cIAP1 was the lowest（12.6%）.The sensitivity,specificity,Youden＇s index,positive predictive value,negative predictive value,and the under-ROC curve area of the combinational application of 12 TAA antibodies mini-array in the detection was 72.4%,91.3%,0.637,94.0%,63.6%,and 0.819,respectively.Conclusion The 12 TAAs mini-array could be a indicator in early diagnosis of lung cancer.The application of 12 TAA antibodies mini-array could be a valuable method with high sensitivity in serological diagnosis of lung cancer.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2012年第4期479-481,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(30872962)
