摘要
目的观察电针对老年痴呆(AD)模型大鼠海马谷氨酸(Glu)含量的影响。方法将SD大鼠按随机数字表分为4组,即正常组、假手术组、模型组、电针组,每组10只。采用双侧海马一次性注射聚集态Aβ25-35制备AD大鼠模型,电针组选取双侧"肾俞"穴、双侧"内关"穴和"大椎"穴治疗6周。采用分光光度法检测各组大鼠海马Glu的变化。结果模型组大鼠海马Glu含量明显高于假手术组,差异有显著性(P<0.01);与模型组相比,电针组大鼠海马Glu含量有明显的下降(P<0.05)。结论电针可能通过降低Glu的神经毒性,保护海马神经细胞,从而改善AD大鼠的学习记忆能力。
Objective To observe the effect of electroacupuncture on the glutamic acid level in the hippocampus of rats with Alzheimer＇s disease（AD）.Methods SD rats were randomly divided into 4 groups： the normal group,sham-operated group,model group,EA group.Bilateral hippocampus were injected condensed Aβ25-35 to be prepared for rat model of AD.Electro-acupuncture group were selected bilateral ＂Shenshu＂ points,bilateral ＂Neiguan＂ points and ＂Dazhui＂ points for 6 weeks,then connected G6805-Ⅱ type of electroacupuncture therapeutic apparatus.We selected the frequence of 2 Hz continuous wave and the intensity of 1mA,last 20min,every day one times,6 times a week,a total of two weeks treatment.We used used spectrophotometric method to detect the changes of Glu in each group.And make a further analysis on the effect of electro-acupuncture＇s intervention to Glu.Results Glu of model group＇s hippocampus were significantly higher than the sham group,and there was a significant difference（P〈0.01）;Compared with model group,Glu of EA group＇s hippocampus decreased significantly（P〈0.05）.Conclusions Its mecha-nism may be achieved through the following means： Reduce Glu neurotoxicity,protect the hippocampal neurons;so as to improve AD rats learning and memory capacity.
出处
《湖北中医药大学学报》
2012年第1期6-8,共3页
Journal of Hubei University of Chinese Medicine
基金
国家自然科学基金资助项目(30772837)
关键词
老年痴呆
电针
海马
谷氨酸
Electro-acupuncture
Alzheimer＇s disease
hippocampal tissue
glutamic acid
