摘要
目的:探讨人卵巢癌细胞A2780中的HES1表达与人卵巢癌侵袭及转移的关系。方法:分别运用实时RT-PCR、WesternBlotting及细胞免疫荧光法检测γ分泌酶抑制剂作用前后A2780细胞中HES1的表达水平,Transwell小室试验及MTT法分别用于检测细胞的侵袭、迁移及黏附能力。结果:下调A2780细胞中HES1的表达水平后,A2780细胞的黏附、侵袭及转移能力下降,差异有统计学意义(P<0.01)。结论:HES1的表达与人卵巢癌细胞的侵袭及转移密切相关,下调其表达可抑制卵巢癌细胞的黏附、侵袭及转移能力。
Objective： To investigate the relationship between HES1 and the invasion and metastasis ability of A2780 cells by down-regulating its expression. Methods： The RNA level of HES 1 was detected by real-time RT-PCR （reverse transcription polymerase chain reaction） method. Western Blotting was used to detect the protein level of HES 1 .The adhesion assay was used to detect the cancer cell adhesion abilities, the invasion and migration abilities were detected by Transwell chambers. Results： After down-regulating the expression of HES 1, the adhesion, invasion and migration abilities of A2780 cells decreased（P 〈 0.01 ）. Conclusion： The expression of HES1 may be correlated with invasion and metastasis potency ofhunman ovary cancer and downregulating its expression can inhibit the adhesion, invasion and migration abilities.
出处
《现代生物医学进展》
CAS
2012年第5期829-832,共4页
Progress in Modern Biomedicine
