摘要
应用聚丙烯酰胺凝胶电泳技术分析了孤雌生殖卤虫产生的罕见雄体、两性卤虫及其杂交后代 (F1)的酯酶 (EST)、超氧物歧化酶 (SOD)、过氧化物酶 (PRX)、碱性磷酸酶 (AL P)、四唑氧化酶(TO)和乳酸脱氢酶 (L DH)的同工酶酶谱表型。对编码 5种酶的 19个位点、35个等位基因的表达进行了遗传分析。结果表明 ,在 EST、PRX和 TO酶谱中既有共显性等位基因 ,又有沉默等位基因的表达 ;卤虫 PRX、AL P和 SOD为单体酶 ,在 EST和 TO酶谱中还存在着二聚体同工酶 ;除 LDH为单态外 ,其余 5种同工酶为多态酶。
The zymogram phenotypes of esterase (EST), superoxide dismutase (SOD), peroxidase (PRX), alkline phosphatase (ALP), tetrazolium oxidase (TO) and lactate dehydrogenase (LDH) in rare males from parthenogenetic Artemia, females of bisexual Artemia and their progeny were determined by using polyacry lamide gel electrophoresis (PAGE). The expression of 35 allozymes at 19 loci coding for EST, SOD, PRX, ALP and TO allozymes was analysed. It was found that there existed both codominant and silent alleles expressed in the zymograms of EST, PRX and TO. The results show that SOD, PRX and ALP isozymes were monosomic, and the discanic isozymes appear in EST and TO zymogram phenotypes of the brine shrimps. It was indicated that LDH was monomrphic, and the other enzymes analysed were polymorphic.
基金
国家自然科学基金课题!(395 70 5 70 )资助
关键词
卤虫
罕见雄体
杂交
等信酶
遗传控制
Artemia
rare males
hydrodization
allozymes
genetic control
