摘要
从日本续断根部的乙醇提取物中分得一个新的三萜皂苷。经过测定,它为:3-O-α-L-吡喃鼠李糖(1→3)-β-D-吡喃葡萄糖(1→3)-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖(常春藤苷元-28-O-β-吡喃葡萄糖酯苷。结果表明,采用一维SEMDY,旋转坐标NOE差谱和选择性远程DEPT核磁共振新技术相结合的方法测定糖链结构不需要对化合物进行化学降解或衍生化,方法简便、快速,测定结果可靠,每个糖基的信号可以分辨和明确归属。
A new 3,28 - bidesmosidic triterpenoid saponin was isolatde from the ethanolic extract of the root of Dipsocus japonicus and its structure established as 3 - 0 - a - L - rhammopyranosyl(1→3) -β - D - glycopranosyl(1 →3) - a - L - rhammopyranosyl ( 1→2) - a - L - arabinopyranosyl - hederagenin -28- 0 - β- D - glycopranoside. The results obtained showed that the chemical structure of oligosaccharide chains of the saponin could be elucidated by the combination of 1D - SEMDY,NOE difference spectroscopy in rotating frame and selective long- range DEFT techniques , without having recourse to chemical degration or modification. Complete assignments of severely overlapping pro-tori resonance of the sugar residues were obtained.
出处
《有机化学》
SCIE
CAS
CSCD
北大核心
2000年第1期81-87,共7页
Chinese Journal of Organic Chemistry
关键词
日本续断
中药
三萜皂苷
化学结构
糖链结构测定
dipsocus japonicus, 3, 28 - bidesmosidic triterpenoid saponin, structural determination of oligosac-charide chains, ROE difference spectroscopy, selective long-range DEFF 13C NMR, ID- SEMDY 1H NMR
