摘要
目的 分析HLA高分辨等位基因与骨髓移植术后HCMVpp65抗原血症的相关性.方法 选取2009年2月至2010年10月在我院行骨髓移植术患者48例；采用免疫组化方法检测患者HCMVpp65,采用直接测序分型方法（PCR-SBT）检测患者HLA-A＊1101、HLA-A＊0201、HLA-A＊2402、HLA-B＊4001、HLA-DRB1＊0901五个高分辨等位基因.结果 ①48例骨髓移植术后患者HCMV感染率100％；②HLA-A＊1101、HLA-A＊0201、HLA-A＊2402、HLA-B＊ 4001等位基因阳性率在pp65抗原血症12例低感染组和36例高感染组中比较没有统计学意义（P＞0.05）,其阳性率HLA-A＊1101为33.3％ （8/24）和20.8％ （15/72）、HLA-A＊0201为4.2％ （1/24）和13.9％ （10/72）、HLA-A＊ 2402为12.5％ （3/24）和19.4％ （14/72）、HLA-B＊ 4001 16.7％ （4/24）和12.5％ （9/72）;③HLA-DRB1＊0901等位基因阳性率在pp65抗原血症12例低感染组和36例高感染组中比较有统计学意义（P =0.048）,其阳性率为4.2％ （1/24）和19.4％ （14/72）；④HLA-DRB1＊0901组患者pp65抗原血症高于HLA-A＊2402组（P =0.007）和HLA-A＊1101组患者（P=0.028）,HLA-A＊0201组患者pp65抗原血症高于HLA-A＊2402组患者（P=0.02）,其他高分辨等位基因组之间pp65抗原血症差异没有统计学意义（P＞0.05）.结论 HLA-DRB1＊0901等位基因可能与骨髓移植术后患者发生高HCMVpp65抗原血症有关；HLA-A＊2402等位基因可能与骨髓移植术后患者发生低HCMVpp65抗原血症有关.
Objective To study the correlation between high-resolution HLA-A ＊ 1101,HLA-A ＊ 0201,HLA-A ＊ 2402,HLA-B ＊ 4001,HLA-DRB1 ＊ 0901 with HCMV pp65 antigenemia after bone marrow transplantation （BMT） in China.Methods 48 recipients doing BMT during 2009.2-2010.10 were selected in my hospital; HCMV pp65 was detected by ELISA or immunohistochemical methods.The frequency of HLA-A ＊ 1101,HLA-A ＊ 0201,HLA-A ＊ 2402,HLA-B ＊ 4001,HLA-DRB1 ＊ 0901 alleles were determined by Polymerase chain reaction-sequence based typing（ PCR - SBT）.Results ① The BMT recipients were HCMV pp65 antigenic positive（ 100% ） ; ② The positive rate of HLA-A ＊ 1101,HLA-A ＊ 0201,HLA-A ＊2402,HLA-B ＊ 4001 showed no obvious difference between 12 lower antigenemia group and 36 higher antigenemia group,the positive rate： HLA-A ＊ 1101 were 33.3% （8/24）and 20.8% （15/72）,HLA-A ＊ 0201 were 4.2% （1/24） and 13.9% （ 10/72）,HLA-A ＊ 2402 were 12.5% （3/24） and 19.4% （ 14/72）,HLA-B ＊ 4001 were 16.7% （4/24） and 12.5% （ 9/72 ） ; ③ HLA-DRB1 ＊ 0901 positive rate in higher antigenemia group was higher than the lower （ P =0.048 ）,the positive rate were 4.2% （ 1/24 ） and 19.4% （14/72） ;④ HLA-DRB1 ＊ 0901 recipients were higher pp65 antigenemia than HLA-A ＊ 2402 recipients （P=0.007） and HLA-A ＊ 1101 recipients （P =0.028）,HLA-A ＊ 0201 recipients were higher pp65 antigenemia than HLA-A ＊ 2402 （ P =0.02）,the pp65 antigenemia showed no obvious difference among the rest of high-resolution HLA groups （P 〉 0.05）.Conclusion HLA-DRB1 ＊ 0901 alleles might be correlated with BMT recipients happened higher pp65 antigenemia,HLA-A ＊ 2402 alleles might be correlated with BMT recipients happened lower pp65 antigenemia.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2011年第6期427-430,共4页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金（30872239）
浙江省卫生部科研基金（WKJ2009-2-023）
关键词
巨细胞病毒
抗原血症
骨髓移植
Cytomegalovirus
Antigenemia
High-resolution HLA
Bone marrow transplantation
