摘要
目的研究荷载白介素24（IL-24）的溶瘤腺病毒ZD55-IL-24联合达卡巴嗪抑制裸鼠黑素瘤的作用。方法建立裸鼠恶性黑素瘤A375细胞移植瘤模型后，分别给予ZD55-IL-24联合达卡巴嗪、ZD55-IL-24、达卡巴嗪、磷酸盐缓冲液（PBS）干预。Western印迹法检测A375细胞移植瘤组织IL-24、E1A蛋白表达。测量裸鼠肿瘤生长体积。结果Western印迹结果表明，ZD55-IL-24联合达卡巴嗪作用的裸鼠黑素瘤高效表达IL-24、E1A蛋白。接种30d后，ZD55-IL-24联合达卡巴嗪组肿瘤平均体积为（2346．5±576．0）mm3，ZD55-IL-24组为（4141．6±1348．2）mm3，达卡巴嗪组为（5230．1±922．8）mm3，PBS组为（7135．1±1002-3）mm3，ZD55-IL-24联合达卡巴嗪组与各组之间差异均有统计学意义（P〈0．05）。结论荷载IL-24基因的溶瘤腺病毒ZD55-IL-24联合达卡巴嗪有抑制黑素瘤增殖的作用。
Objective To investigate the inhibitory effect of dacarbazine and an oncolytic adenovirus carrying interlenkin-24 （IL-24） on transplanted melanoma in nude mice. Methods Nude mice were inoculated with human A375 melanoma cells to establish a model of malignant melanoma. Then, the mice were divided into 4 groups to be treated with an oncolytic adenovirus carrying interleukin-24 （ZD55-IL-24）, dacarbazine, the combination of ZD55-IL-24 and dacarbazine, and phosphate buffer （PBS）, respectively, for 3 days. Seven days after the end of the treatment, some mice were sacrificed followed by the determination of IL-24 and E1A protein levels in tumor tissue by Western blot. The tumor volume was measured on a daily basis for 30 days. Results IL-24 and EIA were highly expressed in melanoma cell-bearing nude mice treated with ZD55-IL=24 and dacarbazine. At 30 days after the inoculation, the average volume of transplanted melanoma was （2346.5 ± 576.0） mm3 in the combination group, significantly different from that in the ZD55-IL-24 group （（4141.6 ± 1348.2） mm3, P 〈 0.05）, dacarbazine group （（5230.1± 922.8） mm3, P 〈 0.05）, and the control group （（7135.1 ± 1002.3） mm3, P 〈 0.05）. Conclusion The ZD55-IL-24 in combination with dacarbazine exhibits a remarkably inhibitory effect on the proliferation of melanoma transplanted into nude mice.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2012年第4期282-283,共2页
Chinese Journal of Dermatology
基金
基金项目：国家自然科学基金（81141102）
徐州市科技发展基金（XF10C064）
