摘要
目的探讨长期饮酒对大鼠内外源性肺损伤时Src抑制的蛋白激酶C底物(Src-suppressed C kinase substrate,SSeCKS)的影响。方法 SD雄性大鼠36只,随机分为单纯饮酒组、饮酒内源性肺损伤组、饮酒外源性肺损伤组、单纯饮水组、饮水内源性肺损伤组和饮水外源性肺损伤组,每组6只。采用实时PCR法测定肺组织SSeCKSmRNA表达变化,比较各组上述指标是否存在差异。结果单纯饮酒组肺组织SSeCKSmRNA表达较饮水组增多(P<0.05),饮酒内、外源性肺损伤各组较饮水内、外源性肺损伤各组增多显著(P<0.05),饮酒内、外源性肺损伤组之间表达无统计学差异(P>0.05)。结论 SSeCKS表达增加是长期饮酒引起急性呼吸窘迫综合征(actue respiratory distress syndrome,ARDS)的易感性增加和病情严重的可能机制之一。
Objective To explore the influnence of chronic alcohol ingestion on the Src-suppressed C kinase substrate（SSeCKS） in rats with acute lung injury caused by pulmonary and extrapulmonary disease.Methods Thirty-six Male Sprague-Dawley rats were randomly divided into six groups：alcohol-drink rats,alcohol-drink and pulmonary ALI rats,alcohol drink and extrapulmonary ALI rats,water-drink rats,water-drink and pulmonary ALI rats,water-drink and extrapulmonary ALI rats.,observe the changs of SSeCKSmRNA in lung were measured by real-time PCR.Results The expressions of SSeCKSmRNA in lung increased in the group of long-term alcohol consumption.（P0.05）.Alcohol-injury groups＇ changes are more significant than water-injury groups.（P0.05）,There was no statistic difference among the extrapulmonary ALI group and pulmonary ALI group（P0.05）.Conclusion The up-regulation of expression of SSeCKSmRNA is a potential mechanism by which chronic alcohol ingestion increased the risk for developing ARDS in critically ill individuals.
出处
《当代医学》
2012年第9期26-28,共3页
Contemporary Medicine
