摘要
目的:探讨不同含巯基氨基酸对人血管内皮细胞的氧化作用。方法:用含有1 000μmol/L的同型半胱氨酸(Hcy)、半胱氨酸(Cys)、还原型谷胱甘肽(GSH)培养液培养细胞24h后,以分光光度比色法分别测定细胞内丙二醛(MDA)含量和超氧化物酶(SOD)活力的改变。结果:Hcy可促使细胞内MDA含量的增加和SOD活力的下降。Cys和GSH对细胞内MDA的含量无影响。Cys可促进细胞内SOD活力的升高。结论:Hcy通过氧化应激促进血管内皮细胞的损伤。
Objective：This study was intended to detail the effects of three thiol amino acids（Hcy,Cys and GSH） on the oxidation of endothelial cells.Methods：Endothelial cells were incubated with Hcy,Cys and GSH（1 000μmol/L）for 24 hours.The contents of MDA and the activities of SOD were measured by chromatometry.Results：Hcy significantly increased the level of MDA and decreased the activities of SOD.Cys increased the activities of SOD.Conclusion：Hcy injuried the endothelial cells through oxidative stress.
出处
《医学理论与实践》
2012年第6期623-624,共2页
The Journal of Medical Theory and Practice
基金
云南省教育厅科学研究基金项目(项目编号08Y0321)
