摘要
利用通过RT-PCR扩增到的M yb28(GenBank注册号:HQ270468)基因分别构建正义和反义植物表达载体,采用冻融法转入农杆菌LBA4404菌株,通过花序浸泡法对Myb28基因缺失的拟南芥进行了遗传转化,经RT-PCR和酶切鉴定,结果表明Myb28正义和反义真核表达载体构建成功,经基因组PCR鉴定表明正义表达载体已成功整合到拟南芥基因组中。
A full-length cDNA encoding Myb28 from Brassica chinensis was isolated using RT-PCR.The recombinant plasmids were transferred into Agrobacterium tunefacien LBA4404 by freeze-thaw method,and then were used to infect mutant Arabidopsis thaliana.Using PCR,we proved that the sense and antisense expression vector were constructed successfully and the genes were integrated into Arabidopsis thaliana.
出处
《生物技术通报》
CAS
CSCD
北大核心
2012年第3期80-84,共5页
Biotechnology Bulletin
基金
国家自然科学基金项目(31101554)
浙江省基金项目(Y3110340)
浙江农林大学启动基金项目(2351001059
2351000812)
关键词
小白菜
M
yb28
硫代葡萄糖苷
表达载体
拟南芥
Brassica.campestris ssp.chinensis var.communis Myb28 Glucosinolates Expression vector Arabidopsis thaliana
