摘要
用高效液相色谱法测定分蘖葱头总黄酮微乳中的槲皮素含量。采用Shmisadezu LC-10ATvp高效液相色谱仪,YMC-PackProC18RS(I.D.250mm×4.6mm)色谱柱,流动相:甲醇-0.4%磷酸溶液(51:49);检测波长:365nm;流速:1.0mL/min。结果表明,槲皮素在0.268～85.816μg/mL范围内线性关系良好,平均回收率98.03%,RSD1.02%。该方法简单、快速、准确、重复性好,可用于测定分蘖葱头总黄酮微乳中的槲皮素含量。
The aim of this study was to determine the contents of quercetin in microemulsion of flavonoid-rich allium cepa L. var. agrogatnm don extract. High performance liquid chromatograph （HPLC） was used in this experiment. The condition as follows： Shmisadezu LC-10A Tvp （250mm×4.6mm） was adopted, mobile phase consisted of methanolwater （51：49）, the wavelength was 365nm, and the flow rate was l.OmL/min. The calibration curve of quercetin was in good linearity with the range of 0.268-85.816 μ g （r=0.999 9）. The average recovery was 98.03%, and RSD was 1.02%. The HPLC method is simple, stable, accurate and reproducible, and it can be used for determining the content of quercetin in microemulsion of flavonoidrich allium cepa L.var. agrogatnm don extract.
出处
《特产研究》
2012年第1期63-65,共3页
Special Wild Economic Animal and Plant Research
基金
吉林省卫生厅项目(20080812)
关键词
分蘖葱头总黄酮
高效液相色谱法
含量测定
Flavonoidrich allium cepa L.var. agrogatnm don extract
HPLC
Determination
