摘要
目的:本论文主要研究抗癌药物卡铂在体外与长度为1341bp的DNA链相互作用的机理。运用原子力显微镜来直接观察卡铂和DNA分子作用后引发的DNA的结构、形貌和长度的变化,来阐明卡铂和DNA的作用方式。方法:卡铂和DNA分子首先在溶液中相互作用,然后取少量作用后的溶液用于原子力显微镜的观察。结果:卡铂与DNA相互作用后,DNA链发生弯曲,长度缩短,且随着浓度提高,长度缩短程度变大。
Objective：In present study,interactions of carboplatin and a linear DNA of 1341bp have been investigated by Atomic force microscopy（AFM）. The changes of DNA structures and the length induced by different concentration of earbopl atin have been investigated. Methods： Carboplatin solution and DNA molecules were mixed, and incubated for different time. The obtained solution was used for preparing AFM sample. Results：The results indicated that both contour length and persistence length of DNA are shortened after interaction with earboplatin.
出处
《黑龙江医药》
CAS
2012年第1期23-24,共2页
Heilongjiang Medicine journal
