摘要
目的:通过建立小鼠体内磨损颗粒颅骨溶解模型,利用显微计算机体层摄影(Micro-computed tomography,Micro-CT)对红霉素和阿仑膦酸钠抑制磨损颗粒诱导小鼠骨溶解进行三维评价,为红霉素阿仑膦酸钠防治人工关节松动提供更多的理论依据。方法:40只BABL/C雌性小鼠随机分为5组。A组为空白对照组;B组为阳性对照组;C组为阿仑膦酸钠组[0.3 mg(/kg.周)];D组红霉素1组[术后7 d腹腔注射红霉素2 mg(/kg.d)];E组红霉素2组[术后腹腔注射红霉素2 mg(/kg.d)];2周后取出颅骨先进行Micro-CT扫描,得到颅骨标本计算机三维图像并进行三维计量分析。然后再进行病理学分析。结果:B组的骨密度(Bone mineral density,BMD)较A组明显降低,C、D、E组的BMD与B组有显著增加。E组的BMD较C、D有明显增高,C、D组间的BMD无统计学意义。B组的骨体积分数(Bone volume fraction,BVF)较A组明显降低,C、D、E组的BVF与B组有显著增加,E组间的BVF较C、D有明显增高,C、D组间的BVF无统计学意义。病理学骨溶解区域破骨细胞数量各组比较结果和BMD的结果类似。结论:红霉素、阿仑膦酸钠可以抑制磨损颗粒诱发的骨溶解。
Objective：To use an in vivo titanium particles induced mouse calvarial osteolysis model to investigate the inhibitory effect of erythromycin and alendronate on the osteolysis.Methods：Forty female eight-week-old BALB/C mice were randomly divided into 5 groups 8 in each group.Control group（A） received sham operation;titanium particle group（B） received 30 mg titanium particle implantation onto the calvariae;alendronate group（C） received 30 mg titanium particle implantation and alendronate 0.3 mg/（kg·weekly） by ip injection;one unit of erythromycin group（D） received 30 mg titanium particle implantation and ip injection of EM 2 mg/（kg·d） in the next week;two units of erythromycin group（E） received ip injection of EM 2 mg/（kg·d） on the next day of 30 mg titanium particles implantation;two weeks later mouse clvariae were harvested.The EM and ALN efficacy was evaluated by Micro-CT and histology.Results：Micro-CT analysis showed that bone mineral density（BMD） and bone volume percentage（BVF） were significantly higher in the EM,ALN treated group（CDE） compared to the Ti group（B）（P0.05）;BMD and BVF were significantly higher in the Control group（A） compared to the Ti group（B）（P0.05）.Histology analysis had a similar result with BMD.Conclusion：Erythromycin and alendronate can effectively inhibite titanium particles induced calvarial osteolysis in vivo.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2012年第1期10-14,共5页
Journal of Chongqing Medical University
基金
国家教育部"春晖计划"合作科研资助项目(编号:Z2008-75008)
关键词
钛颗粒
关节松动
骨溶解
红霉素
阿仑膦酸钠
titanium particle
aseptic loosening
osteolysis
eryt hromycin
alendronate
