摘要
目的探讨屋尘螨(Derp1)抗原对大鼠原代支气管上皮细胞白细胞介素6(IL-6)和IL-8表达的影响。方法将大鼠原代支气管上皮细胞随机分为正常对照组和实验组。实验组再分为3个不同浓度组(1、5及10μg/mL),待细胞生长至融合90%的单层平面时,分别加入不同浓度的Derp1抗原液。在4、8和24 h分别观察各组细胞的形态。采用酶联免疫法(ELISA)检测其细胞上清液IL-6和IL-8的浓度含量。结果正常对照组细胞表现为单层细胞平铺状态,实验组细胞形态及细胞间隙无明显变化,屋尘螨刺激4 h,IL-6和IL-8释放水平开始升高;刺激8 h,IL-6和IL-8继续升高,以5μg/mL组和10μg/mL组更为显著(P<0.01);刺激24 h,IL-6和IL-8进一步升高但与8 h组相比无统计学意义(P>0.05)。结论 Derp1抗原刺激气道上皮细胞释放一系列炎症因子如IL-6和IL-8从而参与哮喘气道炎症反应。
Objective To investigate the effects of dust mite allergen Derp1 on the expressions of IL-6 and IL-8 in primary rat bronchial epithelial cells.Methods The primary rat bronchial epithelial cells were divided into a control group and three experimental groups.In the experimental groups,the cells were cultured with 3 different concentrations of Derp1（1,5,10 μg/mL） for 3 different time（4,8,24 h）.Inverted microscope was employed to observe the morphological changes of bronchial epithelial cells and intercellular space,and supernatants were assayed for IL-6 and IL-8 with ELISA.Results Complete flattening of single cells layer was observed in the control group.In the experimental groups,the cells treated with Derp1 allergen showed no obvious changes in the cell morphology and intercellular space.However,There was a significant change in the level of cytokines production compared with the control group.The levels of IL-6 and IL-8 began to rise at 4 h,and reach to high level at 8 h,especially in the 5 and 10 μg/mL groups（P0.01）.In the 24h group,the concentrations further increased but not reach statistical difference compared with 8h group（P0.05）.Conclusions The Derp1 allergen can stimulate the release of inflammatory cytokines such as IL-6 and IL-8 from the rat trachea-bronchia epithelial cells.It is suggested that dust mite allergen-induced cytokines may play important roles in the pathogenesis of allergic asthma.
出处
《中国呼吸与危重监护杂志》
CAS
2012年第2期116-120,共5页
Chinese Journal of Respiratory and Critical Care Medicine
基金
湖南省医药卫生科研计划项目(编号:B2010-089)
湖南省医药卫生科研计划项目(编号:B2007169)
湖南省科技计划项目(编号:05JT1024)
