摘要
目的观察热休克蛋白27（HSP27）在颞叶癫痫模型大鼠海马CAl区中的表达，探讨HSP27与颞叶癫痫形成的关系。方法健康成年雄性SD大鼠86只，采用随机数字法分为正常对照组（16只）和建模组（70只）；建模组建立氯化锂一匹罗卡品颞叶癫痫大鼠模型，据建模后30d内有无癫痫发作分为颞叶癫痫自发发作组（STLE组）和非自发发作组（n．STLE），正常对照组用生理盐水替代盐酸匹罗卡品，其他处理同建模组。采用匀浆裂解离心提取法分别提取三组大鼠海马CAl区的总蛋白。用免疫印迹法分别检测HSP27在STLE组、n-STLE组和正常组的表达；用免疫电镜观察HSP27在各组海马CAl区的表达。结果免疫印迹法和免疫电镜观察结果相符：在STLE组、n．STLE组和正常组海马CAl区中，HSP27都有表达；免疫印迹相对光密度值分别为0．912±0．011、0．431±0．叭1和0．428±0．010，免疫电镜金颗粒数分别为50．0±4．2、23．0±2．8和20．0±42．3；STLE组中表达最高，与n—STLE组、正常组比较差异有统计学意义（P=0．0001）；n-STLE组和正常组也有表达，但组间比较差异无统计学意义（P=0．63）。结论海马CAl区中HSP27可能参与了颞叶癫痫的形成。
Objective To explore the expression of heat shock protein 27 （HSP27） in the CA1 area of hippocampus in temporal lobe eplepsy （TLE） so as to elucidate the relationship between HSP27 and epleptogenesis of TLE. Methods The model of TLE was induced by lithium-pilocarpine in the experiment group. And the rats were further divided into the STLE and non-STLE groups based upon the absence or presence of recurrent spontaneous seizure in the next 30 days. Total protein fractions from CA1 area of hippocampus were successively obtained through tissue homogenated abstraction. The HSP27 expression in the CA1 area of hippocamp from three groups was semi-quantitatively analyzed by Western blot. And the expression of HSP27 in CA1 area was detected by pre-embedding immunogold electron microscopy. Results Expression of HSP27 in the hippocampus CA1 area as detected by Western blot was in accord with that by immunogold electron microscopy. Relative optical density values were 0. 912 ± 0. 011, 0. 431 ± 0. 011 and 0. 428 ±0. 010 respectively. And gold particles were 50. 0 ±4. 2, 23.0 ±2. 8 and 20. 0 ±2. 3 respectively. The expression of HSP27 was the highest in the hippocampus CA1 area of the STLE group. There was statistical significance as compared with the non-STLE and normal groups （P = 0. 0001 ）. The non-STLE group was higher than the normal group. But there was no significant difference as compared with the normal group （P = 0. 63）. Conclusion HSP27 in the hippocampus CA1 area may participate in the epleptogenesis of TLE.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2012年第9期642-644,共3页
National Medical Journal of China
基金
国家自然科学基金青年基金
关键词
癫痫
颞叶
海马
热休克蛋白类
免疫组织化学
印迹法
蛋白质
Epilepsy, temporal lobe
Hippocampus
Heat-shock proteins
Immunohistochemistry
Blotting,western
