摘要
采用微湿含浸法制备了有序中孔炭/四氧化三铁磁性材料.采用透射电镜和X射线衍射对复合材料进行了表征.将血红蛋白(Hb)固定于材料表面,对其直接电化学行为进行了研究,结果表明Hb在该材料内仍保持了其生物活性,在pH=7.0的PBS缓冲液中,血红蛋白表现出一对峰形良好的准可逆氧化还原峰,为Hb的Fe(Ⅲ)/Fe(Ⅱ)电对的特征峰,求出式电位E0'为-0.306 V,电子转移数为n=1.226,电荷传递系数为α=0.51,表观异相电子转移速率常数为KS=0.0144s-1.在3.00×10-6到1.50×10-4mol/L浓度范围内,血红蛋白的浓度与其响应电流呈良好的线性关系,线性相关系数为R=0.9924,最低检测限为0.270×10-6mol/L.
The novel magnetic composites of ordered mesoporous carbon/Fe304 were prepared with a micro-wetness impregnation method. The composites were characterized by transmission electron microscope and X-ray diffraction. Hemoglobin was immobilized onto the surface of the composites, and was shown to keep its biological activity well. The electrochemical behavior of Hb was studied, and a pair of well-defined, quasi-reversible cyclic voltammetric redox peaks was obtained, which was characteristics of Hb Fe（III）/Fe（II） redox couples. The formal potential （E01）, the electron transfer number （n）, and transfer coefficient （a） were calculated to be 0.306 V, 1.226 and 0.51, respectively. The average apparent heterogeneous electron transfer rate constant （Ks） obtained for Hb was 0.0144 s-1. Linear sweep voltammet- ric measurement of Hb indicated that the peak currents of Hb were linear to its concentration ranging from 3.00 × 10-6 to 1.50 × 10-4 mol/L with the detection limits of 0.270 ×10-6 mol/L.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2012年第5期617-623,共7页
Acta Chimica Sinica
基金
国家自然科学基金(No.20675031)
上海市教委(No.09YZ374)资助项目~~
关键词
有序中孔炭
四氧化三铁
制备
血红蛋白
直接电化学
ordered mesoporous carbon
ferriferrous oxide
preparation
hemoglobin
directelectrochemistry
