摘要
小麦对燕麦孢囊线虫的抗性评价基于根系产生的孢囊数量,为优化小麦抗燕麦孢囊线虫鉴定技术,分别测试了土质类型、接种次数、接种密度、接种苗龄对燕麦孢囊线虫在根系形成孢囊数量的影响。结果表明:燕麦孢囊线虫在砂质粘壤土中产生的孢囊数量最高;对于温麦4号品种,分4次接种处理在小麦根部形成的孢囊数量显著高于分1、2、3次接种处理,但依据传统评价标准,各接种次数处理对其抗性鉴定结果相同,均为高感;每株接1 000头二龄幼虫能较好区分不同抗性程度品种的敏感程度。小麦播后3～6d接种在小麦根部形成的孢囊数量显著高于播种时及播后9～15d接种处理。综合上述结果建议在小麦抗孢囊线虫的鉴定中以采用砂质粘壤土、播后3～6d每株1次性接种1 000二龄幼虫为宜。
Abstract. The criterion of wheat resistance to Heterodera avenae was based on the number of the cysts produced on the roots. To optimize the evaluation technique on wheat resistance toHeterodera avenae e{fect of soil texture, times of inoculation, inoculum densities and seeding age on the number of H. avenae cysts formed on the roots were evaluated in the Lab. The re-sults showed the highest number of cysts was produced in the wheat planted in sandy clay loam; although the number of cysts produced with 4 times of inoculation was higher than thosewith 1--3 times of inoculation, its resistant reaction was not significantly influenced by the times of inoculation based on the resistance criterion. A set of eultivars with different resist-anee could be effectively differentiated at the density of 1000 second stage juveniles （J2） per plant. The numbers of cysts in wheat inoculated in 3-6 d after sowing is higher than those in9- 15 d. We suggest that to obtain an optimum evaluation technique for wheat resistance to H. avenae the wheat should be planted in a sandy clay loam soil and 1000 J2 were inoculated in 3-6 d after sowing.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2012年第1期56-61,共6页
Journal of Hebei Agricultural University
基金
公益性行业科研专项(200903040)
科技部国际合作项目(2009DFB30230)
关键词
燕麦孢囊线虫
土质
抗病性
接种方法
Heterodera avenae
soil texture
resistance
inoculation technique
