摘要
目的探讨细胞因子IFN-γ基因-1615C/T和+5171A/G位点单核苷酸多态性在广西人群中的分布及其对原发性肝细胞癌(HCC)发生、乙型肝炎病毒(HBV)感染的影响。方法设计以医院为基础的病例对照研究,对375名HCC患者、377名HBV携带者和406健康对照进行频数匹配,采用Taq-Man MGB实时荧光定量PCR技术对上述位点进行分型。应用Logistic回归模型分析基因型在三组中的分布差异及基因环境交互作用,并进行连锁不平衡和单倍型分析。结果 -1615C/T和+5171 A/G位点的基因多态性在三组中分布差异无统计学意义(P>0.05)。Logistic回归分析结果显示,吸烟、饮酒和肝癌相关家族史与基因存在交互作用;饮酒联合-1615C/T位点突变型基因T能增加HBV感染风险(OR=1.72,95%CI:1.11～3.26);两个位点的突变型基因T和G联合肝癌相关家族史能增加HCC患病风险(OR:29.24、52.03,95%CI:6.91～123.6、7.02～385.4)。IFN-γ的-1615C/T和+5171A/G位点存在连锁不平衡(D′=0.976,P=2.22-16),但单倍型分布在HCC组与总对照组(HBV携带者对照和健康对照)间无统计学差异。结论IFN-γ的-1615C/T和+5171A/G位点的突变型基因可能不是广西人患HCC和感染HBV的直接危险因素,但环境危险因素对HCC发生和HBV感染有协同作用。
Objective To explore the distribution of cytokines IFN-gamma gene（-1615C/T and ＋5171A/G）single nucleotide polymorphisms in Guangxi people,and the impact of hepatitis B virus（HBV）infection and primary hepatocellular carcinoma（HCC）occurrence.Methods A case-control study based on hospital was carried out and all the objects were frequency matched by 375 HCC patients-377 HBV carriers-406 healthy control.TaqMan MGB Real-Time fluorescence quantitative PCR technology was applied to detect the SNPs of the two loci.The distribution of the genotype and the interaction of gene-environment in the three groups were analyzed by Logistic regression model.The linkage disequilibrium and haplotype of IFN-gamma gene were analyzed.Results There was no significant statistically difference in the polymorphisms of-1615C/T and ＋5171A/G loci among the three groups（P0.05）.There were gene-environment interactions in smoking,alcohol consumption,liver cancer related family history with IFN-gamma gene according to logistic regression analysis.Alcohol consumption combined-1615 locus mutant gene G increased HBV infection risk（OR=1.72,95%CI：1.11~3.26）.The two loci mutant genes combined with liver cancer related family history also enhanced HCC risk（OR：29.24,52.03,95%CI：6.91~123.6,7.02~385.4,respectively）.-1615C/T and ＋5171A/G sites on IFN-gamma had linkage disequilibrium（D′=0.976,P=2.22-16）,but the haplotypes between HCC groups and the total controls（HBV carriers and healthy control）had no significant statistically difference.Conclusion The mutant genes of-1615C/T and ＋5171A/G loci might not influence the occurrence of HCC and HBV infection directly in the population of Guangxi,however they enhanced the risk interacted with the environment risk factors.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第3期329-334,共6页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金资助项目(30860247)
广西自然科学基金资助项目(0832017Z)
广西科学研究与技术开发资助项目(桂科攻0993003D-4)
关键词
原发性肝细胞癌
HBV感染
IFN-Γ
基因多态性
Primary Hepatocellular Carcinoma
HBV infection
Interferon gamma
Gene polymorphism
