摘要
目的建立高效液相色谱法测定阿苯达唑有关物质。方法采用资生堂MGⅡC18柱(250 mm×4.6 mm,5μm)色谱柱;流动相:磷酸二氢铵溶液(1.67 mg.mL 1)-甲醇(40∶60);检测波长:290 nm。结果样品中的杂质均能很好检出,各杂质与主峰之间的分离度良好,检测限为0.228 6 ng。结论该方法简单快速,专属性强,结果准确,可用作阿苯达唑有关物质检查。
OBJECTIVE To establish an HPLC method for determination of related substances in albendazole. METHODS PAK C18 HPLC packed column（MG II, 250 mm × 4.6 mm, 5 um）was used, the mobile phase was ammonium dihydrogen phosphate-methanol（40 ： 60） and the determination wavelength was 290 rim. RESULTS Albendazole and related substances could be well isolated. The detection limit was 0.228 6 ng. CONCLUSION The method is simple, rapid, accurate and suitable for examinating the related substances of albendazole.
出处
《中国现代应用药学》
CAS
CSCD
2012年第3期264-267,共4页
Chinese Journal of Modern Applied Pharmacy
