期刊文献+

A RAPID PCR-QUALITY DNA EXTRACTION METHOD IN FISH

A RAPID PCR-QUALITY DNA EXTRACTION METHOD IN FISH
下载PDF
导出
摘要 PCR has been a general preferred method for biological research in fish,and previous research have enabled us to extract and purify PCR-quality DNA templates in laboratories [1-4].The same problem among these procedures is waiting for tissue digesting for a long time.The overabundance time spent on PCR-quality DNA extraction restricts the efficiency of PCR assay,especially in large-scale PCR amplification,such as SSR-based genetic-mapping construction [5,6],identification of germ plasm resource[7,8] and evolution research [9,10],etc.In this study,a stable and rapid PCR-quality DNA extraction method was explored,using a modified alkaline lysis protocol.Extracting DNA for PCR only takes approximately 25 minutes.This stable and rapid DNA extraction method could save much laboratory time and promotes. PCR has been a general preferred method for biological re- search in fish, and previous research have enabled us to extract and purify PCR-quality DNA templates in laboratories [1-47. The same problem among these procedures is waiting for tissue digesting for a long time. The overabundance time spent on PCR-quality DNA extraction restricts the efficiency of PCR assay, especially in large-scale PCR amplification, such as SSR-based genetic-mapping construction [5, 6], identification of germ plasm resource[7' s] and evolution research [9, 10], etc. In this study, a stable and rapid PCR-quality DNA extraction method was explored, using a modified alkaline lysis protocol. Extracting DNA for PCR only takes approximately 25 minutes. This stable and rapid DNA extraction method could save much laboratory time and promotes.
出处 《水生生物学报》 CAS CSCD 北大核心 2012年第2期365-367,共3页 Acta Hydrobiologica Sinica
基金 Special Fund for Agro-scientific Research in the Public Interest:200903045 Natural Science Foundation of China:31101894
关键词 PCR-quality DNA Fin of fish SSR MITOCHONDRIAL PCR-quality DNA Fin of fish SSR Mitochondrial
  • 相关文献

参考文献2

二级参考文献24

共引文献17

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部