摘要
目的:观察消肿止痛合剂对大鼠急性脊髓损伤的保护作用。方法:采用胥少汀脊髓半横切法制作大鼠T8～T9脊髓损伤模型,根据性别、体质量将60只大鼠随机分为6组,空白组(A),消肿止痛合剂低、中、高剂量组(B、C、D),甲基强的松龙组(E),模型组(F),每组10只,B、C、D组予消肿止痛合剂,每日用药量分别按成人等效剂量的5、10、20倍,每日用药3次,术后第8天开始改为每日2次,剂量不变;E组于术后开始肌注甲基强的松龙(0.03 g/kg);A组和F组分别给予等量生理盐水。给药14天后,用放免法检测血清IL-6、NOS含量;免疫组化法测定Caspase-3的表达;观察各组大鼠HE染色脊髓损伤组织的病理变化。结果:HE染色提示脊髓损伤组织改善情况D组优于C组,且与E组相似;损伤脊髓组织中IL-6、NOS含量F组显著增高,D、C组与E组相比有显著性差异(P<0.01);Caspase-3的表达在F组中升高,C组与E组、D组相比Caspase-3表达有统计学意义(P<0.01)。结论:消肿止痛合剂能降低IL-6、NOS在损伤脊髓组织中的含量,可抑制caspase-3在急性脊髓损伤后的表达。
Objective： To observe the influence of XiaoZhong ZhiTong mixture on rats with acute spinal cord injury. Method： Rat models with T8 -T9 spinal cord injury were established with XU Shao -ring＇ s method of hemi-sectioned spinal cord injury. 60 rats were randomized into blank group （A）, group of XiaoZhong ZhiTong mixture low-dose, medium-dose, high-dose （B, C, D）, methylprednisolone group （E） and model group （F） on average based on gender and physique of the rats. Group of B, C, and D were administered with XiaoZhong ZhiTong mixture of 1/20, 1/10 and 1/5 of adult equivalent dosage, three times per day, then changed to twice per day at the eighth day after the operation; E group were given with intramuscular injection of methylprednisolone（30 mg/kg）. Groups of A and F were given by same amounts of saline. After administration for 14 days, the contents of serum IL-6 and NOS were detected with radioimmunoassay; the expression of Capase-3 was detected with immunohistochemical method. Pathological changes of injured spinal tissues, stained with HE staining, in different groups were observed. Result： HE staining suggested that the improvement of injured spinal cord tissue in D group was superior to that in C group and similar to E group; The contents of IL-6 and NOS in injured spinal cord tissue in F group significantly raised, D and C group compared with E group showed significant difference （P〈0.01）. The expression of Caspase-3 in F group raised, there was statistical meaning of the expression of Caspase-3 in C group compared with E group and D group （P〈0.01）. Conclusion： XiaoZhong ZhiTong mixture can lower the level of IL-6 and NOS in the injured spinal cord tissue and inhibit the expression of caspase-3 after acute spinal cord injury.
出处
《西部中医药》
2012年第3期27-29,共3页
Western Journal of Traditional Chinese Medicine
基金
甘肃省自然科学研究基金项目(编号2009GS00480)
