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免疫组化联合抗体在胸水析出物诊断中的应用 被引量:2

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摘要 目的通过对胸水离心析出物进行石蜡包埋,探讨免疫组化联合抗体在肿瘤细胞学诊断中的应用价值。方法对86例胸水析出物进行石蜡包埋切片,并应用免疫组织化学SP法检测角蛋白(CK5/6、CK8/18)、癌胚抗原(CEA)、波形蛋白(Vim)、间皮细胞(MC)、钙结合蛋白(Cal)和甲状腺转录因子1(TTF-1)的表达并进行统计学分析。结果对胸水析出物进行石蜡包埋切片,联合应用CK5/6、CK8/18、CEA、Vim、MC、Cal和TTF-1七种抗体检测,肿瘤细胞的阳性率与常规细胞学涂片的阳性率之间存在明显的差异(P<0.05),对组织学已经证实的86例恶性肿瘤所致的胸水析出物连续切片,80例为阳性,而在常规涂片中阳性率仅为47例,差异有统计学意义(P<0.05)。结论胸水析出物石蜡包埋切片联合应用免疫组织化学染色,能够明显增加阳性细胞的检出率,具有很高的应用价值。
出处 《河北医药》 CAS 2012年第5期710-711,共2页 Hebei Medical Journal
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