期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

瑞舒伐他汀对人单核-巨噬细胞组织因子表达的影响 被引量：2

Effect and mechanism of rosuvastatin on the expression of tissue factor in cultured human monocyte-macrophages cell induced by oxidized low density lipoprotein
原文传递
导出
摘要 目的探讨瑞舒伐他汀对氧化修饰低密度脂蛋白（OX-LDL）诱导的人单核-巨噬细胞组织因子（TF）表达影响及可能机制。方法据实验要求分空白对照组、OX-LDL组、多聚肌苷酸组、瑞舒伐他汀组。RT-PCR检测各组血凝素样氧化低密度脂蛋白受体-1（LOX-1）mRNA，TFmRNA表达，ELISA检测各组TF蛋白表达。结果OX-LDL组与空白对照组比较，LOX-1mRNA、TFmRNA表达增加[（3．25156±0．15772）VS（1±0）；（2．522451±0．138967）VS（1±0）]，其差异均有统计学意义（P〈0．01）。多聚肌苷酸组、瑞舒伐他汀组与OX-LDL组比较，LOX-1mRNA、TFmRNA表达减少[（2．95139±0．157253）VS（3．25156±0．15772）、（2．877343±0．156558）VS（3．25156±0．15772）；（1．811956±0．169699）VS（2．522451±0．138967）、（1．687701．4-0．174647）vs（2．522451±0．138967）]，其差异均有统计学意义（P〈0．05）。ox-LDL组与空白对照组比较，TF蛋白表达增加[（207．7233±1．154701）VS（184．8467±0．871799）]，差异有统计学意义（P〈0．01）。多聚肌苷酸组、瑞舒伐他汀组与OX-LDL组比较，TF蛋白表达均减少[（197．8733±1．505003）vs（207．72334-1．154701）、（202．95674-2．722744）VS（207．7233±1．154701）]，差异均有统计学意义（P〈0．05）。结论LOX-1可能介导OX-LDL诱导的人单核-巨噬细胞TF表达增加。瑞舒伐他汀可通过下调单核-巨噬细胞LOX．1mRNA的表达下调TFmRNA表达，从而下调TF蛋白表达。 Objective To investigate the effects and mechanism of rosuvastatin on the expression of tissue factor in cultured human monocyte-macrophages cells which was induced by oxidized low density lipo- protein（ox-LDL）. Methods The human monocyte-macrophages cells were divided into four groups： con- trol group, ox-LDL group, Poly-inosine monophosphate （Poly I ）group, rosuvastatin group. The expressionof LOX-lmRNA and TF mRNA was assayed by RT-PCR. The ELISA was performed to determine the protein concentration of TF. Results Compared with control group, the expression of LOX-1 mRNA and TF mR- NA was increased in the ox-LDL group [ （ 3.25156 ± 0. 15772 ） vs （ 1 ± 0 ） ; （2. 522451 ± 0.138967 ） vs （ 1 ±0） ], and it was in a concentration-dependent manner （ P 〈 0. 01 ）. Compared with the expression ofLOX-1 mRNA in the Poly-inosine monophosphate group and rosuvastatin group, TF mRNA were decreased in the ox-LDL group[ （2. 95139 ±0. 157253） vs（3.25156 ±0. 15772） ; （2. 877343 ±0. 156558） vs（3. 25156 ± 0. 15772） ; （ 1. 811956 ± 0. 169699 ） vs （2. 522451 ± 0. 138967 ） ; （ 1. 687701 ± 0. 174647 ） vs （2. 522451 ± 0. 138967）], and if was in a concentration-dependent manner（ P 〈 0. 05）. Compared with control group,the expression of TF in the ox-LDL group was increased [ （ 207. 7233 ± 1. 154701 ） vs （ 184. 8467 ± 0. 871799） ], and it was in a concentration-dependent manner （ P 〈0. 01 ）. Compared with the Poly-ino- sine monophosphate group and rosuvastatin group [ （ 197. 8733 ± 1. 505003） vs （207.7233 ± 1. 154701 ） ;（202. 9567 ±2. 722744）vs（207. 7233 ±1. 154701 ）] , the expression of TF in the ox-LDL group were de- creased, and it was in a concentration-dependent manner （ P 〈 0. 05 ）. Conclusions LOX-1 may be re- sponsible for the expression of TF in human monocyte-macrophages cells induced by ox-LDL. Rosuvastatin is able to down-regulate the expression of LOX-lmRNA in human monoeyte-maerophages cells through ox- L
作者 宋海彬 张羽 关坤萍 申晓彧 赵丰
机构地区 山西医科大学第二医院心血管内科
出处 《中国医师杂志》 CAS 2012年第2期173-176,共4页 Journal of Chinese Physician
关键词 氟苯类/药理学 嘧啶类/药理学 磺胺类/药理学 单核巨噬细胞系统/代谢/药物作用 凝血致活酶/代谢 Fluorobenzenes/PD Pyrimidines/PD Sulfonamides/PD Mononuclear phagocytesystem/ME/DE Thromboplastin/ME
分类号 R9 [医药卫生—药学]
  • 相关文献

参考文献3

二级参考文献42

  • 1沈杨.急性冠脉综合征患者血清hs-CRP水平及辛伐他汀的早期干预探讨[J].中国医师杂志,2004,6(9):1267-1268. 被引量：1
  • 2罗玉梅,万新红,姜德谦,文丹,楚罗湘,郭洪波.辛伐他汀干预对急性冠脉综合征患者血清白细胞介素- 6、高敏C反应蛋白的影响(英文)[J].中国现代医学杂志,2005,15(15):2245-2249. 被引量：3
  • 3Rossig L, Dimmeler S, Zeiher AM. Apoptosis in the vascular wall and atherosclerosis. Basic Res Cardiol,2001,96 ( 1 ) : 11-22. 被引量：1
  • 4Kockx M, Knaapen MW. The role of apoptosis in vascular disease. J Pathol, 2000,190 ( 3 ) : 267-280. 被引量：1
  • 5Li DY, Mehta JL. Antisense to LOX-1 inhibits oxidized LDL-mediated upregulation of monocyte chemoattractant protein-1 and monocyte adhesion to human coronary artery endothelial cells. Circulation,2000,101 ( 25 ) :889- 895. 被引量：1
  • 6Dayuan Li, Jawahar L. Mehta. Upregulation of Endothelial Receptor for Oxidized LDL ( LOX-1 ) by Oxidized LDL and Implications in Apoptosis of Human Coronary Artery Endothelial Cells. Artefioscler Thromb Casc Biol,2000,20 : 1116-1122. 被引量：1
  • 7Raif P. Roads to Dysfunction : Argininase Ⅱ Contributes to Oxidized Low- Density Lipoprotein-Induced Attenuation of Endothelial NO Production. Circulation Research, 99 ( 9 ) : 918 -920, October 27, 2006. 被引量：1
  • 8Chen XP,Xun KL,Wu Q,et al. Oxidized low density lipoprotein receptor- 1 mediates oxidized low density lipoprotein-induced apoptosis in human umbilical cells:role of reactive oxygen species. Vascul Pharmacol,2007, 47(1) :1-9. 被引量：1
  • 9Inoue N ,Sawamura T. Lectin-hke oxidized LDL receptor-1 as extracelluar chaperone receptor: its versatile functions and human disease. Methods, 2007,43 (3) :218-222. 被引量：1
  • 10Vakkilainen J, Steiner G, Ansquer JC, et al. Relationships between lowdensity lipo protein particle size, plasma lipoproteins, and progression of coronary artery disease :the Diabetes Atherosclerosis Intervention Study (DAIS). Circulation ,2003,107 ( 13 ) : 1733-1737. 被引量：1

共引文献346

同被引文献32

  • 1万东,罗勇,谢鹏.电刺激小脑顶核对缺血／再灌注大鼠脑组织内NF-κB活性及其活化的影响[J].中华物理医学与康复杂志,2006,28(10):660-665. 被引量：18
  • 2Detrano RC, Wong ND, Doherty TM,et al. Prognostic significance of coronary calcific deposits in asymptomatic high-risk subjects [J] . Am J Med, 1997,102: 344-349. 被引量：1
  • 3Goodman WG, Goldin J, Kuizon BD, et al . Coronary-artery calcification in young adults with end-stage renal disease who are undergoing dialysis [ J]. N Engl J Med, 2000, 342: 1 478-483. 被引量：1
  • 4Wagenknecht LE, Bowden DW, Carr JJ, et al. Familial aggregation of coronary artery calcium in families with type 2 diabetes [ J ]. Diabetes, 2001, 50: 861-866. 被引量：1
  • 5Mohler ER III, Gannon F, Reynolds C, et al. Bone formation and inflammation in cardiac valves [J]. Circulation, 2001, 103: 1 522- 528. 被引量：1
  • 6Cowell SJ, NewbyDE, Prescott RJ, et al. Arandomizedtrial of intensive lipid-lowering therapy in calcific aortic stenosis [ J ]. N Engl J Med, 2005, 352 (23) : 2 389-397. 被引量：1
  • 7Raison J, Rudnichi A, Safar ME. Effects of atorvastatin on aortic pulse wave velocity in patients with hypertension and hypercholesterolaemia , a preliminary study [ J ]. J Hum Hypertens 2002, 16: 705-710. 被引量：1
  • 8Luigi Giusto Spagnoli, Elena Bonanno, Giuseppe Sangiorgi, et al. Role of Inflammation in Atherosclerosis [ J]. J N ucl Med, 2007, 48: 1 800-815. 被引量：1
  • 9Joseph Shalhoub, Mika A Falck-Hansen, Alun H Davies, et al. Innate immunity and monocyte-macrophage activation in atherosclerosis[J]. Journal of Inflammation, 2011, 8(9): 1-17. 被引量：1
  • 10Chang K, Weiss D, Suo J, Vega JD, Giddens D, Taylor WR, Jo H. Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and atherosclerosis [ J ]. Circulation, 2007, 116: 1 258 -266. 被引量：1

引证文献2

二级引证文献17

中国医师杂志
2012年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部