摘要
目的探讨不同程度失神经支配后面神经眼支和口支的超微结构变化。方法建立豚鼠右侧面神经阻断15 s、30 s和切断的动物模型。于术后1周和1个月切取面神经眼支和口支,应用半薄切片和超薄切片在光镜和电镜下观察面神经眼支和口支的病理变化,探讨失神经支配后面神经眼支和口支病理变化过程及两者差异。结果光镜下半薄切片测定面神经眼支和口支的直径,正常情况下,面神经眼支直径大于口支直径;当神经受损伤1周时,面神经平均直径变小,眼支始终大于口支。当神经受损伤1个月时,15 s 1个月组面神经口支直径大小有所恢复,接近正常,但眼支恢复较差;30 s1个月组,面神经眼支和口支均没有明显恢复。电镜下面神经眼支和口支不同损伤程度的超微结构比较,当神经受损伤1周时,随着损伤程度加重,电镜下面神经眼支和口支超微结构改变加重,15 s 1周<30 s 1周<切断1周。当损伤1月时,神经功能有一定的恢复,电镜下面神经眼支和口支超微结构损伤减轻,15 s 1周>15 s 1个月,30 s 1周>30 s 1个月;眼支大于口支,面神经口支较眼支易恢复。面神经切断组,神经远端没有任何恢复,病变程度加重。结论面神经眼支直径大于口支,面神经眼支较口支易受损伤,且较口支不易恢复,面神经受压榨时间越长,损伤越重,恢复越差。
Objective To explore the ultrastructural changes of experimentally denervated and reinnervated guinea pigs facial nerve.Methods The animal model was set up in three groups that the right facial nerve was resected and compressed for 15 seconds and 30 seconds respectively in 30 guinea-pigs.At two sequential intervals of 1 week and 1 month,the eye-branch and mouth-branch of facial nerve were excised on both sides.we discuss the pathological changes on both branches of facial nerve and the difference between them by light microscope（LM） in half-thin section and by electron microscope（EM） in ultrathin section when the facial nerve were injuried.Results The diameter and observe the morphology of the eye-branch and mouth-branch of facial nerve were calcuated by LM in half-thin section.In normal,the diameter of the eye-branch is bigger than that of mouth-branch.When the facial nerve is injuried for 1 week,the diameter of facial nerve become smaller,the diameter of the eye-branch is bigger than that of mouth-branch always.When the facial nerve is injuried for 1 month,in the group of 15s the diameter of mouth-branch of facial nerve become bigger and near normal,but the eye-branch recover badly.In the group of 1 month 30 s,neither eye-branch nor mouth-branch of facial nerve recover apparently.To compare the ultrastructure of eye-branch of facial nerve with mouth-branch in EM in different degree of compression.When the facial nerve is injuried for 1 week,the ultrastructure damage of eye-branch and mouth-branch of facial nerve is badly in EM,15s 30 s excision group.When the facial nerve is injuried for 1 month,the function of facial nerve recover in certain extent,the ultrastructure damage of eye-branch and mouth-branch of facial nerve is alleviated in EM,15 s 1 week15 s 1 month,30 s 1 week30 s 1 month,eye-branch mouth-branch,mouth-branch is easier to recover than eye-branch.In the group of excision,the terminal of nerval axon hasn＇t recover a little,the damage degree becomes worse.Conclusion The diameter of the eye
出处
《解剖学研究》
CAS
2012年第1期35-39,共5页
Anatomy Research
基金
辽宁省自然科学基金(20072097)
