摘要
目的分析淋巴瘤患者外周血中循环microRNA-21(miR-21)的表达,探讨循环miR-21在淋巴瘤早期诊断中的意义。方法收集60例临床确诊的淋巴瘤患者外周血,50名健康体检人群外周血作为正常对照组,用FQ-PCR方法检测miR-21的表达;同时检测对应的临床确诊淋巴瘤患者肿瘤组织及外周血中miR-21的表达,判断二者的相关性。结果 FQ-PCR检测结果显示,淋巴瘤患者外周血中循环miR-21的表达显著高于正常对照组,差异有统计学意义(t=6.9,P<0.000 1);且淋巴瘤患者外周血中循环miR-21的表达与肿瘤组织中miR-21的表达密切相关(r2=0.931,P<0.000 1)。结论外周血循环miR-21检测有望成为淋巴瘤早期诊断的新的分子标志。
Objective To study the expression of serum circulating miR-21 in the patient with lymphoma and to validate its sig nificance in early diagnosis of lymphoma. Methods We detected serum circulating miR21 expression by FQPCR in lymphoma patients （n=60） and health controls（n= 50）. At the same time, we detected miR-21 expression by FQ PCR in matched tumor tissue and serum from diffuse large B cell lymphoma Cymphoma patients（n=30）. Results FQ PCR results indicated miR-21 higher ex- pression in serum from Cymphoma patients than controls （t= 6.9, P〈0. 0001）, and peripheral circulating miR-21 expression levels had very close correlation with those that matched tumor tissue in lyrnphoma cases （r2 =0. 931 ,P〈0. 0001）. Conclusion Circulating miR 21 may be expected to be as a new biomarker to early detection of lymphoma.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第6期535-536,539,共3页
Chongqing medicine
基金
国家自然科学基金资助项目(81071921)
武汉市科技攻关计划项目(201062938366-02)
