摘要
目的:考察氯新酮乳膏的体外抑菌活性和对昆明种小鼠白念珠菌性阴道炎的疗效。方法:体外实验采用试管二倍稀释法测定氯新酮乳膏对白念珠菌、大肠杆菌、铜绿假单孢菌和金黄色葡萄球菌的最低抑菌浓度(MIC)。体内实验:在昆明种小鼠阴道内注入白念珠菌混悬液(5×106个/ml)建造阴道炎模型,分别以氯新酮乳膏和10%洁尔阴洗液治疗,对小鼠的阴道灌洗液做涂片镜检,并测定真菌载量,观察小鼠阴道的体征变化。结果:氯新酮乳膏在体外实验中对白念珠菌、大肠杆菌、铜绿假单孢菌和金黄色葡萄球菌的MIC分别为1.6、6.25、1.6和3.125mg/ml。小鼠的阴道体征、阴道灌洗液涂片镜检和真菌载量检查结果表明,氯新酮对白念珠菌性阴道炎的疗效优于洁尔阴(P<0.05)。结论:氯新酮乳膏对阴道炎致病菌有很好的抑制活性,对小鼠白念珠菌性阴道炎有明显的治疗作用。
Objective： To investigate the antibacterial activities and therapeutic effect of Lüxintong cream on Candida albicans vaginitis in Kunming mice.Methods：The minimum inhibitory concentrations（MIC） of Lüxintong cream for C.albicans,Escherichia coli,Pseudomonas aeruginosa and Staphylococcus aureus were measured by tube double dilution method in vitro.Suspension with 5×106 /ml C.albicans was injected into vagina of the mice to establish vaginitis models.The mice in treatment group and control group were treated with Lüxintong cream and 10% Jieeryin lotion,respectively.The vagina irrigating solution was smeared and observed by microscope and fungus colony was counted.Results：The MIC values of Lüxintong cream for C.albicans,E.coli,P.aeruginosa and S.aureus were 1.6,6.25,1.6 and 3.125 mg/ml,respectively.Physical characteristics of vagina,observation of vagina irrigating solution and colony count of C.albicans all showed that Lüxintong cream had better therapeutic effects than Jieeryin lotion.Conclusion：Lüxintong cream has antibacterial activities and therapeutic effects on C.albicans vaginitis of the mice.
出处
《药学服务与研究》
CAS
CSCD
2012年第1期49-51,共3页
Pharmaceutical Care and Research
关键词
氯新酮
洁尔阴洗液
白念珠菌
阴道炎
小鼠
Lüxintong cream； Jieeryin lotion； Candida albicans； vaginitis； mice
