摘要
目的:探讨RNA干扰技术沉默Survivin基因对鼻咽癌细胞CNE-2恶性表型的影响。方法:设计、合成针对Survivin基因的干扰序列,并将干扰序列构建至pGCsi/U6/GFP载体,稳定转染CNE-2细胞;逆转录聚合酶链反应(RT-PCR)、Western blot鉴定干扰效果;经四甲基偶氮唑蓝(MTT)法、流式细胞术、平板克隆形成实验分析沉默Survivin基因后,CNE-2细胞生长速度、细胞周期及恶性表型的改变。结果:沉默Survivin基因后,CNE-2细胞Sur-vivin mRNA、蛋白质表达水平均明显下调;细胞生长速度下降,G1期细胞增多,S期细胞减少,克隆形成能力明显减弱。结论:Survivin基因在CNE-2细胞生长、细胞周期及恶性表型等方面都起着重要的作用。
Aim：To investigate the effects of RNAi-mediated gene silence of Survivin on the prolifera-tion and malignant phenotype of nasopharyngeal carcinoma cells. Methods： Nasopharyngeal carcinomacell line CNE-2 was transfected by Survivin small interfering RNA （siRNA） and the efficacy of Survivinsilencing was determined with RT-PCR and Western blot. The growth and the malignant phenotype ofCNE-2 was examined by tetrazolium blue （MTI？） assay, flow cytometry and colony formation assay. Re-suits： The level of Survivin gene in CNE-2 was obviously declined after transfected with survivin siRNA.The cell growth rate and clone forming ability was significantly decreased. Morever, the distribution ofcell cycles of CNE-2 cells was changed. Conclusion：Survivin gene plays an important role in prolifera-tion, cell cycle and the malignant phenotype of CNE-2.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2011年第6期607-610,656,共5页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
国家自然科学基金资助项目(30901663)
