摘要
为建立谷子突变体库,以便在谷子分子生物学研究中应用,以生产上主要推广种植的谷子品种‘长农35号’干种子为实验材料,采用0.8%和1.0%甲磺酸乙酯(EMS)进行诱变处理,试验共收获株型相关M1代突变体材料282份,其中,0.8%EMS诱变‘长农35号’获得株型突变体材料100个单株,可分为10个组;1.0%EMS诱变‘长农35号’获得株型突变体材料182个单株,可分为17个组。M1代成熟期株型相关性状突变体分析结果表明:1.0%EMS处理‘长农35号’所获突变体,在株高、穗下节粗、穗下第一节间粗和茎节数4个性状与对照有显著差异,而0.8%处理与对照差异不显著。因此,对于‘长农35号’来讲,采用1.0%EMS进行诱变处理更有利于多类型大量突变单株的获得。
Foxtail millet cultivar ‘ Changnong35 ＇,which is widely used in agricultural production,was treated with EMS to establish foxtail millet mutant library for the molecular biology applications.We obtained 282 mutants in the M 1 generation related to plant type,of which,100 mutant plants obtained by using 0.8% EMS treatment can be divided into 10 groups;182 mutant plants obtained by using 1.0% EMS treatment can be divided into 17 groups.The analysis results of the mature plant type traits of the M 1 generation showed thatplant height,stem diameter under spike,the diameter of the first internode and internode numbers treated with 1.0% EMS comparing with the control were significantly different,while the 0.8% EMS treatment comparing with control was not significant,therefore,the treatment of 1.0% EMS was more conducive to obtainment of a large number and multi-type mutants for ＇ Changnong35 ＇.
出处
《中国农学通报》
CSCD
北大核心
2011年第18期84-89,共6页
Chinese Agricultural Science Bulletin
基金
山西省农科院博士基金项目"EMS诱变谷子品种长农35号突变体库的建立与应用研究"(YBSJJ1001)
国家谷子现代农业产业技术体系谷子栽培植保项目
