摘要
研究了不同流速下4种溶质在两种不同反相色谱柱上的色谱图及它们的动力学色谱性质,进一步验证了溶质相对保留值(RRT)与流动相流速之间存在良好的双对数线性关系,并对溶质在流速改变时洗脱顺序发生改变的现象从动力学因素上进行了合理的解释;同时还发现色谱填料的孔径及拓扑学构型不同将导致溶质在其上具有不同的动力学色谱参数及动力学色谱行为。
Different chromatograms of four solutes were obtained by reversed-phase liquid chromatography （RPLC） with two chromatographic columns at different flow rates. The dynamic chromatographic behavior of solutes in RPLC was studied and the results confirmed that the logarithm of the relative retention time （RRT） of solutes was proportional to the logarithm of the flow rate of mobile phase （v）. The reason for that the elution order changed with change of the flow rate of mobile phase was explained reasonable with dynamic factors of solutes. We also found that the solutes would have different dynamic chromatographic parameters and behaviors if the pore-size and topology configuration of chromatographic packings were not the same.
出处
《分析试验室》
CAS
CSCD
北大核心
2012年第3期66-69,共4页
Chinese Journal of Analysis Laboratory
基金
陕西省自然科学基金项目(2006B28)资助
关键词
流速
液相色谱
洗脱顺序
保留值
Flow rate
Liquid chromatography
Elution order
Retention value
