摘要
基于在pH 8.00磷酸氢二钠-柠檬酸缓冲介质中,血红蛋白对过氧化氢氧化对甲酚反应具有强的催化作用,汞(Ⅱ)对上述指示反应具有灵敏的抑制作用,从而提出了抑制荧光光谱法测定模拟废水中汞含量的方法。优化的试验条件如下:①血红蛋白的浓度为2.00×10-7 mol·L-1;②对甲酚的浓度为1.60×10-3 mol·L-1;③过氧化氢的浓度为1.11×10-3 mol·L-1;④反应时间为80min。在激发波长318nm、发射波长405nm处,反应体系的ΔF(即F0与F值之差)与汞(Ⅱ)的浓度在2.00×10-8～1.00×10-5 mol·L-1范围内呈线性关系。方法用于模拟废水的分析,测得加标回收率在94.0%～105%之间,相对标准偏差(n=8)在2.5%～3.3%之间。
It was found that in Na2 HPO4 citric acid buffer medium of pH 8. 00, the oxidation of p-cresol by hydrogen peroxide was strongly catalyzed by haemoglobin （Hb）, leading to an enhancement of fluorescence. The indicator reaction was inhibited by the presence of Hg（ Ⅱ ）. Based on these facts, the determination of trace amount of Hg（ Ⅱ ） in simulated wastewater by inhibitory spectrofluorimetry was proposed. The optimized conditions found were as follows： ① concentration of Hb： 2.00X 10 rmol · L^-1 ; ② concentration of p-cresol： 1.60× 10-amol · L^-1 ; ③ concentration of H2O2 ： 1. 11× 10 Smol · L^-1 ④ time of reaction： 80 min. Linear relationship was found between values of △SF（F0-F） determined at wavelengths of 318 nm （λex） and 405 nm （λem） and concentration of Hg（ Ⅱ ） in the range of 2. 00 × 10^-8-1.00 5〈 10^-5 mol · L^-1. The proposed method was used in the analysis of simulated wastewater, giving values of recovery and RSD＇s （n=8） in the ranges of 94. 0%-105% and 2. 5%-3. 3%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2012年第2期207-209,212,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家自然科学基金(NO.20973087)
关键词
抑制荧光光谱法
模拟废水
汞
Keywords： Inhibitory fluorophotometry
Simulated wastewater
Mercury
